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Identification of Differentially Expressed Genes Involved in the Molecular Mechanism of Pericarp Elongation and Differences in Sucrose and Starch Accumulation between Vegetable and Grain Pea (Pisum sativum L.)
- Source :
- International Journal of Molecular Sciences, Volume 20, Issue 24
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- Pea (Pisum sativum L.), as a major source of plant protein, is becoming one of the major cultivated crop species worldwide. In pea, the pericarp is an important determinant of the morphological characteristics and seed yield. To investigate the molecular mechanism of pericarp elongation as well as sucrose and starch accumulation in the pods of different pea cultivars, we performed transcriptomic analysis of the pericarp of two types of pea cultivar (vegetable pea and grain pea) using RNA-seq. A total of 239.44 Gb of clean sequence data were generated, and were aligned to the reference genome of Pisum sativum L. In the two samples, 1935 differentially expressed genes (DEGs) were identified. Among these DEGs, three antioxidant enzyme superoxide dismutase (SOD) were detected to have higher expression levels in the grain pea pericarps at the pod-elongating stages. Otherwise, five peroxidase (POD)-encoding genes were detected to have lower expression levels in the vegetative pericarps at the development stage of pea pod growth. Furthermore, genes related to starch and sucrose metabolism in the pea pod, such as SUS, INV, FBA, TPI, ADPase, SBE, SSS, and GBSS, were found to be differentially expressed. The RNA-seq data were validated through real-time quantitative RT-PCR of 13 randomly selected genes. Our findings provide the gene expression profile of, as well as differential expression information on, the two pea cultivars, which will lay the foundation for further studies on pod development and nutrition accumulation in the pea and provide valuable information for pea cultivar improvement.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Sucrose
Starch
pea (Pisum sativum L.)
01 natural sciences
Catalysis
Article
Pisum
Inorganic Chemistry
03 medical and health sciences
chemistry.chemical_compound
Sativum
Gene expression
Cultivar
Physical and Theoretical Chemistry
gene
Molecular Biology
Gene
Spectroscopy
biology
pod elongation
Organic Chemistry
Peas
food and beverages
General Medicine
biology.organism_classification
Computer Science Applications
Horticulture
030104 developmental biology
Point of delivery
chemistry
Gene Expression Regulation
Plant protein
Seeds
RNA-seq
transcriptome
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 20
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a9759f282dfaaf5fb54f08334c29af76