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Probing the epigenetic signatures in subjects with coronary artery disease
- Source :
- Molecular Biology Reports. 47:6693-6703
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Depletion of S-adenosyl methionine and 5-methyltetrahydrofolate; and elevation of total plasma homocysteine were documented in CAD patients, which might modulate the gene-specific methylation status and alter their expression. In this study, we have aimed to delineate CAD-specific epigenetic signatures by investigating the methylation and expression of 11 candidate genes i.e. ABCG1, LIPC, PLTP, IL-6, TNF-α, CDKN2A, CDKN2B, F2RL3, FGF2, P66 and TGFBR3. The methylation-specific PCR and qRT-PCR were used to assess the methylation status and the expression of candidate genes, respectively. CAD patients showed the upregulation of IL-6, TNF-α, CDKN2A, CDKN2B, F2RL3, FGF2, P66, and TGFBR3. Hypomethylation of CDKN2A loci was shown to increase risk for CAD by 1.79-folds (95% CI 1.22–2.63). Classification and regression tree (CART) model of gene expression showed increased risk for CAD with F2RL3 > 3.4-fold, while demonstrating risk reduction with F2RL3
- Subjects :
- Adult
Male
0301 basic medicine
Oncology
medicine.medical_specialty
Candidate gene
Homocysteine
Coronary Artery Disease
Folic Acid Deficiency
Epigenesis, Genetic
03 medical and health sciences
chemistry.chemical_compound
Folic Acid
0302 clinical medicine
Downregulation and upregulation
Risk Factors
CDKN2A
Internal medicine
CDKN2B
Diabetes Mellitus
Genetics
medicine
Humans
Epigenetics
Correlation of Data
Molecular Biology
Cyclin-Dependent Kinase Inhibitor p16
Cyclin-Dependent Kinase Inhibitor p15
Demography
Interleukin-6
Tumor Necrosis Factor-alpha
business.industry
General Medicine
Methylation
DNA Methylation
Middle Aged
F2RL3
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Regression Analysis
Female
Fibroblast Growth Factor 2
Proteoglycans
Receptors, Thrombin
business
Receptors, Transforming Growth Factor beta
Biomarkers
Subjects
Details
- ISSN :
- 15734978 and 03014851
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Molecular Biology Reports
- Accession number :
- edsair.doi.dedup.....a98a1bb9a5ef73c7c59afeb7fb845209