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Quantification of **Candida albicans** by flow cytometry using TO-PRO®-3 iodide as a single-stain viability dye

Authors :
Monique Kerstens
Paul Cos
Louis Maes
Peter Delputte
Mario Hellings
Liesbeth Voeten
Isabel Pintelon
Gaëlle Boulet
Source :
Journal of microbiological methods
Publication Year :
2013

Abstract

This study investigated a flow cytometric, single-stain approach to quantify Candida albicans as an alternative for the standard viable plate count. TO-PRO®-3 iodide allows an excellent distinction between viable and dead cells. Both quantification methods show a high degree of correlation.

Details

Language :
English
ISSN :
01677012
Database :
OpenAIRE
Journal :
Journal of microbiological methods
Accession number :
edsair.doi.dedup.....a98fd3bc4224f54ee14805c6fd78478d