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High-Resolution Micro-object Separation by Rotating Magnetic Chromatography

Authors :
Jishou Piao
Lu Liu
Long Cai
Hyok Chol Ri
Xiangzi Jin
Huaze Sun
Xiangfan Piao
Hai-bo Shang
Xuejun Jin
Qiaosheng Pu
Yong Cai
Zhongping Yao
Donatella Nardiello
Maurizio Quinto
Donghao Li
Source :
Analytical chemistry. 94(33)
Publication Year :
2022

Abstract

The development of new technologies for the separation, selection, and isolation of microparticles such as rare target cells, circulating tumor cells, cancer stem cells, and immune cells has become increasingly important in the last few years. Microparticle separation technologies are usually applied to the analysis of disease-associated cells, but these procedures often face a cell separation problem that is often insufficient for single specific cell analyses. To overcome these limitations, a highly accurate size-based microparticle separation technique, herein called "rotating magnetic chromatography", is proposed in this work. Magnetic nanoparticles, placed in a microfluidic separation channel, are forced to move in well-defined trajectories by an external magnetic field, colliding with microparticles that are in this way separated on the basis of their dimensions with high accuracy and reproducibility. The method was optimized by using fluorescein isothiocyanate-modified polystyrene particles (chosen as a reference standard) and then applied to the analysis of cancer cells like Hep-3B and SK-Hep-1, allowing their fast and high-resolution chromatographic separation as a function of their dimensions. Due to its unmatched sub-micrometer cell separation capabilities, RMC can be considered a break-through technique that can unlock new perspectives in different scientific fields, that is, in medical oncology.

Details

ISSN :
15206882
Volume :
94
Issue :
33
Database :
OpenAIRE
Journal :
Analytical chemistry
Accession number :
edsair.doi.dedup.....a994d246623b632182446c4dcee560c1