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Abrogation of the RNase activity of Erns in a low virulence classical swine fever virus enhances the humoral immune response and reduces virulence, transmissibility, and persistence in pigs
- Source :
- Virulence, Vol 12, Iss 1, Pp 2037-2049 (2021), Virulence, article-version (VoR) Version of Record, IRTA Pubpro. Open Digital Archive, Institut de Recerca i Tecnologia Agroalimentàries (IRTA), Wang, Miaomiao; Bohórquez, José Alejandro; Hinojosa, Yoandry; Muñoz-González, Sara; Gerber, Markus; Coronado, Liani; Perera, Carmen Laura; Liniger, Matthias; Ruggli, Nicolas; Ganges, Llilianne (2021). Abrogation of the RNase activity of Erns in a low virulence classical swine fever virus enhances the humoral immune response and reduces virulence, transmissibility, and persistence in pigs. Virulence, 12(1), pp. 2037-2049. Taylor & Francis 10.1080/21505594.2021.1959715
- Publication Year :
- 2021
- Publisher :
- Taylor & Francis Group, 2021.
-
Abstract
- The prevalence of low virulence classical swine fever virus (CSFV) strains makes viral eradication difficult in endemic countries. However, the determinants for natural CSFV attenuation and persistence in the field remain unidentified. The aim of the present study was to assess the role of the RNase activity of CSFV Erns in pathogenesis, immune response, persistent infection, and viral transmission in pigs. To this end, a functional cDNA clone pPdR-H30K-36U with an Erns lacking RNase activity was constructed based on the low virulence CSFV field isolate Pinar de Rio (PdR). Eighteen 5-day-old piglets were infected with vPdR-H30K-36U. Nine piglets were introduced as contacts. The vPdR-H30K-36U virus was attenuated in piglets compared to the parental vPdR-36U. Only RNA traces were detected in sera and body secretions and no virus was isolated from tonsils, showing that RNase inactivation may reduce CSFV persistence and transmissibility. The vPdR-H30K-36U mutant strongly activated the interferon-α (IFN-α) production in plasmacytoid dendritic cells, while in vivo, the IFN-α response was variable, from moderate to undetectable depending on the animal. This suggests a role of the CSFV Erns RNase activity in the regulation of innate immune responses. Infection with vPdR-H30K-36U resulted in higher antibody levels against the E2 and Erns glycoproteins and in enhanced neutralizing antibody responses when compared with vPdR-36U. These results pave the way toward a better understanding of viral attenuation mechanisms of CSFV in pigs. In addition, they provide novel insights relevant for the development of DIVA vaccines in combination with diagnostic assays for efficient CSF control. info:eu-repo/semantics/publishedVersion
- Subjects :
- Microbiology (medical)
RNase P
Swine
Immunology
Virulence
610 Medicine & health
Infectious and parasitic diseases
RC109-216
Microbiology
Virus
Classical Swine Fever
viral persistence
Immune system
Ribonucleases
Interferon
medicine
Animals
viral attenuation
Neutralizing antibody
pestivirus
630 Agriculture
biology
viral transmission
biology.organism_classification
Virology
erns rnase activity
Immunity, Humoral
Infectious Diseases
Viral replication
Classical swine fever
Classical Swine Fever Virus
biology.protein
570 Life sciences
590 Animals (Zoology)
viral replication
Parasitology
Persistent Infection
type i ifn
classical swine fever virus (csfv)
humoral response
medicine.drug
Research Article
Research Paper
Subjects
Details
- Language :
- English
- ISSN :
- 21505608 and 21505594
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Virulence
- Accession number :
- edsair.doi.dedup.....a9a9b21c34e84845c5345316754c7776