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Kidney Gene Expression Analysis in a Rat Model of Intrauterine Growth Restriction Reveals Massive Alterations of Coagulation Genes
- Source :
- Endocrinology. 148:5549-5557
- Publication Year :
- 2007
- Publisher :
- The Endocrine Society, 2007.
-
Abstract
- In this study, low birth weight was induced in rats by feeding the dams with a low-protein diet during pregnancy. Kidneys from the fetuses at the end of gestation were collected and showed a reduction in overall and relative weight, in parallel with other tissues (heart and liver). This reduction was associated with a reduction in nephrons number. To better understand the molecular basis of this observation, a transcriptome analysis contrasting kidneys from control and protein-deprived rats was performed, using a platform based upon long isothermic oligonucleotides, strengthening the robustness of the results. We could identify over 1800 transcripts modified more than twice (772 induced and 1040 repressed). Genes of either category were automatically classified according to functional criteria, making it possible to bring to light a large cluster of genes involved in coagulation and complement cascades. The promoters of the most induced and most repressed genes were contrasted for their composition in putative transcription factor binding sites, suggesting an overrepresentation of the AP1R binding site, together with the transcription induction of factors actually binding to this site in the set of induced genes. The induction of coagulation cascades in the kidney of low-birth-weight rats provides a putative rationale for explaining thrombo-endothelial disorders also observed in intrauterine growth-restricted human newborns. These alterations in the kidneys have been reported as a probable cause for cardiovascular diseases in the adult.
- Subjects :
- medicine.medical_specialty
Intrauterine growth restriction
Biology
Kidney
Rats, Sprague-Dawley
Transcriptome
Endocrinology
Pregnancy
Internal medicine
Gene expression
medicine
Animals
Gene Regulatory Networks
Gene
Oligonucleotide Array Sequence Analysis
Fetal Growth Retardation
Gene Expression Profiling
Gene Expression Regulation, Developmental
Promoter
Complement System Proteins
medicine.disease
Blood Coagulation Factors
Rats
DNA binding site
Gene expression profiling
Disease Models, Animal
medicine.anatomical_structure
Female
Subjects
Details
- ISSN :
- 19457170 and 00137227
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- Endocrinology
- Accession number :
- edsair.doi.dedup.....a9b2ca335bf4fd182ce1b3b547a6a0c3