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MicroRNA-106b functions as an oncogene in renal cell carcinoma by affecting cell proliferation, migration and apoptosis
- Source :
- Molecular medicine reports. 13(2)
- Publication Year :
- 2015
-
Abstract
- Kidney cancer is the 14th most common cancer in the world and its prognosis remains poor due to difficult early detection and treatment. Therefore, the identification of biomarkers for early-stage renal cell carcinoma (RCC) is important. MicroRNA-106b (miR-106b) has been described as an oncogene in several types of human cancer. Previous microarray studies have suggested that miR-106b was significantly upregulated in RCC tissues compared with paired normal kidney tissues and may be a promising biomarker for the prediction of early metastasis following nephrectomy. The present study aimed to determine the expression and function of miR-106b in RCC. The expression of miR-106b in RCC tissues and cells, and in paired normal tissues and cells was determined by reverse transcription quantitative polymerase chain reaction, based on the previous sequencing results of miRNAs. Furthermore, a wound scratch assay, MTT assay and flow cytometry were performed to examine the functions of miR-106b on cell migration, proliferation and apoptosis. The results demonstrated that miR-106b was upregulated in RCC tissues and cell lines compared with control normal tissues and cell lines. Downregulation of miR-106b with a synthesized inhibitor suppressed cell migration and proliferation and induced renal cancer cell apoptosis, suggesting that miR-106b can be characterized as an oncogene in RCC. To the best of our knowledge, the present study was the first to reveal that miR-106b is upregulated and affects cellular migration, proliferation and apoptosis in RCC. Further studies are required to examine the role and target genes of miR-106b in RCC.
- Subjects :
- 0301 basic medicine
Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Cell
Down-Regulation
Apoptosis
Biology
urologic and male genital diseases
Transfection
Biochemistry
03 medical and health sciences
0302 clinical medicine
Renal cell carcinoma
Cell Movement
Cell Line, Tumor
microRNA
Genetics
medicine
Humans
Molecular Biology
Carcinoma, Renal Cell
Aged
Cell Proliferation
Oncogene
Cell growth
Cancer
Cell migration
Oncogenes
Cell cycle
Middle Aged
medicine.disease
Flow Cytometry
Kidney Neoplasms
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
Female
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 13
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....a9b40c90a0a7f2922a7b5781f32611ea