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Functional characterization of an abiotic stress-inducible transcription factor AtERF53 in Arabidopsis thaliana
- Source :
- Plant Molecular Biology. 82:223-237
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- AP2/ERF proteins play crucial roles in plant growth and development and in responses to biotic and abiotic stresses. ETHYLENE RESPONSE FACTOR 53 (AtERF53) belongs to group 1 in the ERF family and is induced in the early hours of dehydration and salt treatment. The functional study of AtERF53 is hampered because its protein expression in Arabidopsis is vulnerable to degradation in overexpressed transgenic lines. Taking advantage of the RING domain ligase1/RING domain ligase2 (rglg1rglg2) double mutant in which the AtERF53 can express stably, we investigate the physiological function of AtERF53. In this study, we demonstrate that expression of AtERF53 in wild-type Arabidopsis was responsive to heat and abscisic acid (ABA) treatment. From results of the cotransfection experiment, we concluded that AtERF53 has positive transactivation activity. Overexpression of AtERF53 in the rglg1rglg2 double mutant conferred better heat-stress tolerance and had resulted in higher endogenous ABA and proline levels compared to rglg1rglg2 double mutants. AtERF53 also has a function to regulate guard-cell movement because the stomatal aperture of AtERF53 overexpressed in rglg1rglg2 double mutant was smaller than that in the rglg1rglg2 double mutant under ABA treatment. In a global gene expression study, we found higher expressions of many stress-related genes, such as DREB1A, COR15A, COR15B, PLC, P5CS1, cpHSC70 s and proline and ABA metabolic-related genes. Furthermore, we identified several downstream target genes of AtERF53 by chromatin immunoprecipitation assay. In conclusion, the genetic, molecular and biochemical result might explain how AtERF53 serving as a transcription factor contributes to abiotic stress tolerance in Arabidopsis.
- Subjects :
- Transcriptional Activation
Hot Temperature
Proline
Ubiquitin-Protein Ligases
Mutant
Arabidopsis
Plant Science
Biology
Response Elements
Transactivation
chemistry.chemical_compound
Plant Growth Regulators
Gene Expression Regulation, Plant
Stress, Physiological
Genetics
Arabidopsis thaliana
Abscisic acid
Transcription factor
Oligonucleotide Array Sequence Analysis
Arabidopsis Proteins
Reverse Transcriptase Polymerase Chain Reaction
Abiotic stress
Gene Expression Profiling
fungi
food and beverages
General Medicine
Plants, Genetically Modified
biology.organism_classification
Molecular biology
Cell biology
Isoenzymes
chemistry
Mutation
Plant Stomata
Trans-Activators
Agronomy and Crop Science
Chromatin immunoprecipitation
Abscisic Acid
Protein Binding
Transcription Factors
Subjects
Details
- ISSN :
- 15735028 and 01674412
- Volume :
- 82
- Database :
- OpenAIRE
- Journal :
- Plant Molecular Biology
- Accession number :
- edsair.doi.dedup.....a9e232ebb181f9a9aa2438cf4392ec66