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A plant-specific module for homologous recombination repair

A plant-specific module for homologous recombination repair

Authors :
Xuanpeng Wang
Lili Wang
Yongchi Huang
Zhiping Deng
Cunliang Li
Jian Zhang
Mingxi Zheng
Shunping Yan
Source :
Proceedings of the National Academy of Sciences of the United States of America. 119(16)
Publication Year :
2022

Abstract

Homologous recombination repair (HR) is an error-free DNA damage repair pathway to maintain genome stability and a basis of gene targeting using genome-editing tools. However, the mechanisms of HR in plants are still poorly understood. Through genetic screens for DNA damage response mutants (DDRM) in Arabidopsis, we find that a plant-specific ubiquitin E3 ligase DDRM1 is required for HR. DDRM1 contains an N-terminal BRCT (BRCA1 C-terminal) domain and a C-terminal RING (really interesting new gene) domain and is highly conserved in plants including mosses. The ddrm1 mutant is defective in HR and thus is hypersensitive to DNA-damaging reagents. Biochemical studies reveal that DDRM1 interacts with and ubiquitinates the transcription factor SOG1, a plant-specific master regulator of DNA damage responses. Interestingly, DDRM1-mediated ubiquitination promotes the stability of SOG1. Consistently, genetic data support that SOG1 functions downstream of DDRM1. Our study reveals that DDRM1-SOG1 is a plant-specific module for HR and highlights the importance of ubiquitination in HR.

Details

ISSN :
10916490
Volume :
119
Issue :
16
Database :
OpenAIRE
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Accession number :
edsair.doi.dedup.....a9f93e042ac4e8f688c0cc5c2f17809d