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Use of EST-SSR Markers for Evaluating Genetic Diversity and Fingerprinting Celery (Apium graveolens L.) Cultivars
- Source :
- Molecules, Vol 19, Iss 2, Pp 1939-1955 (2014), Molecules; Volume 19; Issue 2; Pages: 1939-1955, Molecules
- Publication Year :
- 2014
- Publisher :
- MDPI AG, 2014.
-
Abstract
- Celery (Apium graveolens L.) is one of the most economically important vegetables worldwide, but genetic and genomic resources supporting celery molecular breeding are quite limited, thus few studies on celery have been conducted so far. In this study we made use of simple sequence repeat (SSR) markers generated from previous celery transcriptome sequencing and attempted to detect the genetic diversity and relationships of commonly used celery accessions and explore the efficiency of the primers used for cultivars identification. Analysis of molecular variance (AMOVA) of Apium graveolens L. var. dulce showed that approximately 43% of genetic diversity was within accessions, 45% among accessions, and 22% among horticultural types. The neighbor-joining tree generated by unweighted pair group method with arithmetic mean (UPGMA), and population structure analysis, as well as principal components analysis (PCA), separated the cultivars into clusters corresponding to the geographical areas where they originated. Genetic distance analysis suggested that genetic variation within Apium graveolens was quite limited. Genotypic diversity showed any combinations of 55 genic SSRs were able to distinguish the genotypes of all 30 accessions.
- Subjects :
- Genotype
celery
EST-SSR
genetic diversity
fingerprint
genotypic diversity
Pharmaceutical Science
Biology
Analysis of molecular variance
Article
Analytical Chemistry
lcsh:QD241-441
lcsh:Organic chemistry
Drug Discovery
Genetic variation
Botany
Cultivar
Physical and Theoretical Chemistry
Apium
Expressed Sequence Tags
Molecular breeding
Genetic diversity
Polymorphism, Genetic
Organic Chemistry
UPGMA
Genetic Variation
Apium graveolens
DNA Fingerprinting
Genetic distance
Chemistry (miscellaneous)
Molecular Medicine
Microsatellite Repeats
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Volume :
- 19
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....aa02f42ff08c8db04e971e83ce2271e7