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In depth analysis of apoptosis induced by silica coated manganese oxide nanoparticles in vitro
- Source :
- Journal of Hazardous Materials. 283:519-528
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Manganese oxide nanoparticles (MnO NPs) have been regarded as a new class of T1-positive contrast agents. The cytotoxicity of silica coated MnO NPs (MnO@SiO2 NPs) was investigated in human cervical carcinoma cells (HeLa) and mouse fibroblast cells (L929). The changes of cell viability, cell morphology, cellular oxidative stress, mitochondrial membrane potential and cell cycle induced by MnO@SiO2 NPs were evaluated. Compared to HeLa cells, L929 cells showed lower cell viability, more strongly response to oxidative stress and higher percentage in the G2/M phase of cell cycle. The appearance of sub-G1 peak, double staining with Annexin V-FITC/PI and the increase of Caspase-3 activity further confirmed apoptosis should be the major form of cell death. Moreover, the apoptotic pathway was clarified as follows. Firstly, reactive oxygen species (ROS) is generated induced by MnO@SiO2 NPs, then p53 is activated followed by an increase in the bax and a decrease in the bcl-2, ultimately leading to G2/M phase arrest, increasing the activity of caspase-3 and inducing apoptosis.
- Subjects :
- Programmed cell death
Environmental Engineering
Health, Toxicology and Mutagenesis
Apoptosis
Cell morphology
medicine.disease_cause
Cell Line
HeLa
Mice
medicine
Animals
Humans
Environmental Chemistry
Viability assay
Waste Management and Disposal
bcl-2-Associated X Protein
Membrane Potential, Mitochondrial
chemistry.chemical_classification
Reactive oxygen species
biology
Caspase 3
Chemistry
Cell Cycle
Oxides
Fibroblasts
Cell cycle
Silicon Dioxide
biology.organism_classification
Pollution
Oxidative Stress
Manganese Compounds
Proto-Oncogene Proteins c-bcl-2
Biochemistry
Biophysics
Nanoparticles
Tumor Suppressor Protein p53
Reactive Oxygen Species
Oxidative stress
HeLa Cells
Subjects
Details
- ISSN :
- 03043894
- Volume :
- 283
- Database :
- OpenAIRE
- Journal :
- Journal of Hazardous Materials
- Accession number :
- edsair.doi.dedup.....aa066cab499a820b2d8873577166108d