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Genome-wide analysis, expression profile of heat shock factor gene family (CaHsfs) and characterisation of CaHsfA2 in pepper (Capsicum annuum L.)
- Source :
- BMC Plant Biology
- Publisher :
- Springer Nature
-
Abstract
- Background Heat shock factors (Hsfs) play crucial roles in plant developmental and defence processes. The production and quality of pepper (Capsicum annuum L.), an economically important vegetable crop, are severely reduced by adverse environmental stress conditions, such as heat, salt and osmotic stress. Although the pepper genome has been fully sequenced, the characterization of the Hsf gene family under abiotic stress conditions remains incomplete. Results A total of 25 CaHsf members were identified in the pepper genome by bioinformatics analysis and PCR assays. They were grouped into three classes, CaHsfA, B and C, based on highly conserved Hsf domains, were distributed over 11 of 12 chromosomes, with none found on chromosome 11, and all of them, except CaHsfA5, formed a protein–protein interaction network. According to the RNA-seq data of pepper cultivar CM334, most CaHsf members were expressed in at least one tissue among root, stem, leaf, pericarp and placenta. Quantitative real-time PCR assays showed that all of the CaHsfs responded to heat stress (40 °C for 2 h), except CaHsfC1 in thermotolerant line R9 leaves, and that the expression patterns were different from those in thermosensitive line B6. Many CaHsfs were also regulated by salt and osmotic stresses, as well as exogenous Ca2+, putrescine, abscisic acid and methyl jasmonate. Additionally, CaHsfA2 was located in the nucleus and had transcriptional activity, consistent with the typical features of Hsfs. Time-course expression profiling of CaHsfA2 in response to heat stress revealed differences in its expression level and pattern between the pepper thermosensitive line B6 and thermotolerant line R9. Conclusions Twenty-five Hsf genes were identified in the pepper genome and most of them responded to heat, salt, osmotic stress, and exogenous substances, which provided potential clues for further analyses of CaHsfs functions in various kinds of abiotic stresses and of corresponding signal transduction pathways in pepper. Electronic supplementary material The online version of this article (doi:10.1186/s12870-015-0512-7) contains supplementary material, which is available to authorized users.
- Subjects :
- CaHsfA2
Osmotic shock
Amino Acid Motifs
Molecular Sequence Data
Plant Science
Sodium Chloride
Biology
Genes, Plant
Chromosomes, Plant
chemistry.chemical_compound
Heat Shock Transcription Factors
Plant Growth Regulators
Gene Expression Regulation, Plant
Osmotic Pressure
Sequence Analysis, Protein
Pepper
Gene Duplication
Gene family
Amino Acid Sequence
Protein Interaction Maps
Gene
Conserved Sequence
Phylogeny
Plant Proteins
Cell Nucleus
Genetics
Identification of CaHsfs family
Methyl jasmonate
Abiotic stress
Gene Expression Profiling
Gene Expression Regulation, Developmental
food and beverages
Adaptation, Physiological
Protein Structure, Tertiary
DNA-Binding Proteins
Gene expression profiling
Heat shock factor
chemistry
Organ Specificity
Multigene Family
Gene expression
Capsicum
Genome, Plant
Research Article
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 14712229
- Volume :
- 15
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology
- Accession number :
- edsair.doi.dedup.....aa07c50ff4db817224e96327d89295bb
- Full Text :
- https://doi.org/10.1186/s12870-015-0512-7