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UbcH10 overexpression may represent a marker of anaplastic thyroid carcinomas
- Source :
- British Journal of Cancer 93 (2005): 464–471. doi:10.1038/sj.bjc.6602721, info:cnr-pdr/source/autori:P Pallante1, MT Berlingieri1, G Troncone2, M Kruhoffer3, TF Orntoft3, G Viglietto1, A Caleo2, I Migliaccio2, M Decaussin-Petrucci4, M Santoro1, L Palombini2 and A Fusco*,1,5/titolo:UbcH10 overexpression may represent a marker of anaplastic thyroid carcinomas./doi:10.1038%2Fsj.bjc.6602721/rivista:British Journal of Cancer/anno:2005/pagina_da:464/pagina_a:471/intervallo_pagine:464–471/volume:93, Pallante, P, Berlingieri, M T, Troncone, G, Kruhøffer, M, Ørntoft, T F, Viglietto, G, Caleo, A, Migliaccio, I, caussin-Petrucci, M, Santoro, M, Palombini, L & Fusco, A 2005, ' UbcH10 overexpression may represent a marker of anaplastic thyroid carcinomas ', Br. J. Cancer, vol. 93 ., British Journal of Cancer
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- The hybridisation of an Affymetrix HG_U95Av2 oligonucleotide array with RNAs extracted from six human thyroid carcinoma cell lines and a normal human thyroid primary cell culture led us to the identification of the UbcH10 gene that was upregulated by 150-fold in all of the carcinoma cell lines in comparison to the primary culture cells of human normal thyroid origin. Immunohistochemical studies performed on paraffin-embedded tissue sections showed abundant UbcH10 levels in thyroid anaplastic carcinoma samples, whereas no detectable UbcH10 expression was observed in normal thyroid tissues, in adenomas and goiters. Papillary and follicular carcinomas were only weakly positive. These results were further confirmed by RT–PCR and Western blot analyses. The block of UbcH10 protein synthesis induced by RNA interference significantly reduced the growth rate of thyroid carcinoma cell lines. Taken together, these results would indicate that UbcH10 overexpression is involved in thyroid cell proliferation, and may represent a marker of thyroid anaplastic carcinomas.
- Subjects :
- endocrine system
Cancer Research
Pathology
medicine.medical_specialty
carcinomas
endocrine system diseases
Blotting, Western
Mice, Transgenic
Biology
anaplastic
thyroid
Thyroid carcinoma
Mice
Biomarkers, Tumor
Tumor Cells, Cultured
Carcinoma
medicine
Animals
Humans
Thyroid Neoplasms
Anaplastic carcinoma
Molecular Diagnostics
Thyroid cancer
Cell Proliferation
Reverse Transcriptase Polymerase Chain Reaction
Cell growth
Thyroid
UbcH10
medicine.disease
Rats
Up-Regulation
Gene Expression Regulation, Neoplastic
Cell Transformation, Neoplastic
medicine.anatomical_structure
Oncology
Cell culture
Ubiquitin-Conjugating Enzymes
immunohistochemistry
Cancer research
Immunohistochemistry
cell cycle
Subjects
Details
- ISSN :
- 15321827 and 00070920
- Volume :
- 93
- Database :
- OpenAIRE
- Journal :
- British Journal of Cancer
- Accession number :
- edsair.doi.dedup.....aa0cbad9fde6c9105d7dc8278451d6e0