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Inhibitory Effects of Purple Sweet Potato Leaf Extract on the Proliferation and Lipogenesis of the 3T3-L1 Preadipocytes
- Source :
- The American Journal of Chinese Medicine. 43:915-925
- Publication Year :
- 2015
- Publisher :
- World Scientific Pub Co Pte Ltd, 2015.
-
Abstract
- Purple sweet potato leaves (PSPLs) are healthy vegetable that is rich in anti-oxidants. A solution of boiling water extract of PSPL (PSPLE) is believed to be able to prevent obesity and metabolic syndrome in the countryside of Taiwan, but its efficacy has not yet been verified. The purpose of this study was to investigate the possible anti-adipogenesis effect of PSPLE in vitro. PSPLE was used to treat the 3T3-L1 cells, and the effects on cell proliferation and adipogenesis were investigated. The results showed that PSPLE caused a dose-dependent decrease in the cell proliferation of 3T3-L1 preadipocytes, but did not alter the cell viability. In addition, PSPLE induced ERK inactivation in the 3T3-L1 preadipocytes. Furthermore, pre-treatment of confluent 3T3-L1 cells with PSPLE led to reduced lipid accumulation in differentiated 3T3-L1 cells. The inhibition of lipogenesis could result from the PSPLE-induced down-regulation of the expression of the C/EBPα and SREBP-1 transcription factors during 3T3-L1 adipocyte differentiation. These results suggest that PSPLE not only inhibits cell proliferation at an early stage but also inhibits adipogenesis at a later stage of the differentiation program.
- Subjects :
- Cell Survival
MAP Kinase Signaling System
Cellular differentiation
Down-Regulation
Gene Expression
Biology
Mice
chemistry.chemical_compound
3T3-L1 Cells
Adipocyte
Adipocytes
CCAAT-Enhancer-Binding Protein-alpha
Animals
Obesity
Viability assay
Ipomoea batatas
Cell Proliferation
Metabolic Syndrome
Adipogenesis
Dose-Response Relationship, Drug
Plant Extracts
Cell growth
Lipogenesis
Water
Cell Differentiation
3T3-L1
General Medicine
Cell biology
Plant Leaves
Complementary and alternative medicine
Biochemistry
chemistry
Sterol Regulatory Element Binding Protein 1
Phytotherapy
Subjects
Details
- ISSN :
- 17936853 and 0192415X
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- The American Journal of Chinese Medicine
- Accession number :
- edsair.doi.dedup.....aa174d629038ca8c6658727940548d06