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Genetic Heterogeneity of Borrelia burgdorferi in the United States
- Source :
- Journal of Infectious Diseases. 175:98-107
- Publication Year :
- 1997
- Publisher :
- Oxford University Press (OUP), 1997.
-
Abstract
- To examine in detail Borrelia burgdorferi strain diversity in the United States, 186 isolates from human, tick, and rodent sources were analyzed from multiple distinct geographic regions of the United States and abroad. Strains were characterized by genomic macrorestriction analysis and ospA and 23S rDNA gene sequencing followed by phylogenetic analysis. Results indicate that spirochetal isolates from the United States fall into two major divisions and nine or more subdivisions; human isolates fell into five of these subdivisions. Greater genetic diversity was observed among B. burgdorferi isolates from moderate climatic regions, consistent with increased tick vector and reservoir diversity. All of the Borrelia isolates were reactive by ospA polymerase chain reaction except for Borrelia hermsii controls and several tick isolates from the Northeast, which were shown to lack the 49-kb plasmid encoding outer surface protein A (OspA). The data suggest that US B. burgdorferi isolates demonstrate substantial genetic heterogeneity, with regional differences in spirochete populations.
- Subjects :
- Disease reservoir
Climate
Lipoproteins
Rodentia
DNA, Ribosomal
Polymerase Chain Reaction
Genetic Heterogeneity
Borrelia burgdorferi Group
Borrelia
Animals
Humans
Immunology and Allergy
Borrelia burgdorferi
Phylogeny
Disease Reservoirs
Geographic difference
Genetics
Lyme Disease
Genetic diversity
Ixodes
biology
Genetic Variation
bacterial infections and mycoses
biology.organism_classification
United States
Electrophoresis, Gel, Pulsed-Field
Bacterial vaccine
RNA, Bacterial
RNA, Ribosomal, 23S
Infectious Diseases
Genes, Bacterial
Antigens, Surface
Bacterial Vaccines
Lyme disease microbiology
Arachnid Vectors
Borrelia hermsii
Polymorphism, Restriction Fragment Length
Bacterial Outer Membrane Proteins
Subjects
Details
- ISSN :
- 15376613 and 00221899
- Volume :
- 175
- Database :
- OpenAIRE
- Journal :
- Journal of Infectious Diseases
- Accession number :
- edsair.doi.dedup.....aa22e73745d08e6bbf647c45eb4cdb19
- Full Text :
- https://doi.org/10.1093/infdis/175.1.98