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First molecular characterization of Sarcocystis miescheriana in wild boars (Sus scrofa) from Latvia
- Source :
- Parasitology Research. 119:3777-3783
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Various muscle samples of wild boar (Sus scrofa) from Latvia were studied for the presence of Sarcocystis infection by means of morphological and molecular methods. Sarcocysts were detected in 122 out of 140 (87.1%) wild boar examined. According to the morphological appearance of sarcocysts, the observed cysts belonged to one morphological type and resembled Sarcocystis miescheriana. Twenty-three sarcocysts isolated from the muscles of Latvian wild boars were molecularly characterized at 18S rRNA, ITS1 and cox1. Additionally, eight sarcocysts obtained from Lithuanian wild boars were subjected to molecular analysis in order to compare intraspecific genetic variability. The amplified 18S rRNA region using newly designed primers is sufficiently variable to separate S. miecheriana from S. suihominis. All Latvian and Lithuanian isolates were confirmed belonging to S. miescheriana. No genetic variation was detected within 18S rRNA and ITS1. By contrast, the high intraspecific genetic variability of S. miescheriana was observed within cox1 since each newly obtained sequence represented a unique haplotype. The comparison made using S. miescheriana isolates from Italian and Japanese wild boar and Chinese domestic pig revealed the genetic similarity of the samples depending on their geographical distances. The current study provides the first detection of Sarcocystis infection in wild boars from Latvia and molecular characterization of S. miescheriana.
- Subjects :
- Sarcocystosis
Population genetics
Swine
Sus scrofa
030231 tropical medicine
Zoology
Wild boar
Polymerase Chain Reaction
Intraspecific competition
18S ribosomal RNA
030308 mycology & parasitology
03 medical and health sciences
0302 clinical medicine
Species Specificity
biology.animal
Genetic variation
RNA, Ribosomal, 18S
Animals
Genetic variability
Phylogeny
0303 health sciences
General Veterinary
biology
Muscles
Haplotype
Sarcocystis
General Medicine
DNA, Protozoan
biology.organism_classification
Latvia
cox1
Domestic pig
Infectious Diseases
Haplotypes
Insect Science
Sarcocystis miescheriana
Parasitology
Subjects
Details
- ISSN :
- 14321955 and 09320113
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Parasitology Research
- Accession number :
- edsair.doi.dedup.....aa4b466badc1d2c371bc77020205da28