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The immune response elicited by mammary adenocarcinoma cells transduced with interferon-gamma and cytosine deaminase genes cures lung metastases by parental cells
- Source :
- Human gene therapy. 9(2)
- Publication Year :
- 1998
-
Abstract
- The parental cells of the TSA murine mammary adenocarcinoma (TSA-pc) were transfected with both the interferon-gamma (IFN-y) gene and the cytosine deaminase (CD) suicide gene to obtain a therapeutic vaccine active against TSA-pc lung metastases. Even in the absence of treatment with the prodrug 5-fluorocytosine (5-FC), the local growth of double transfectants (CD-y clones) was inhibited by a marked recruitment of granulocytes and macrophages. In mice harboring TSA-pc micrometastases, therapeutic vaccination with either IFN-gamma or CD single transfectants reduced the number of lung nodules, whereas CD-gamma double transfectants abrogated metastasis growth in up to 80% of mice. Treatment of mice with 5-FC did not alter the curative efficacy of CD-gamma double-transfectant cells. By contrast, in mice vaccinated with CD single-transfectant cells, 5-FC treatment caused a significant loss of their curative activity. Host T cells played an active role in the cure of lung metastases, because vaccination of nude mice with CD-gamma cells was uneffective.
- Subjects :
- Cytotoxicity, Immunologic
Lung Neoplasms
viruses
Mice, Nude
Nucleoside Deaminases
Biology
Adenocarcinoma
Transfection
immune response
Metastasis
Cytosine Deaminase
Interferon-gamma
Mice
Immune system
Interferon
Genetics
medicine
Animals
Molecular Biology
Mice, Inbred BALB C
Lung
gene therapy
lung metastasis
Cytosine deaminase
Immunotherapy, Active
Mammary Neoplasms, Experimental
Genetic Therapy
Suicide gene
medicine.disease
Vaccination
medicine.anatomical_structure
Immunology
Cancer research
Molecular Medicine
Female
Neoplasm Transplantation
medicine.drug
T-Lymphocytes, Cytotoxic
Subjects
Details
- ISSN :
- 10430342
- Volume :
- 9
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Human gene therapy
- Accession number :
- edsair.doi.dedup.....aa7ba192526b63532110411f9a9e508f