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Cytokinesis of neuroepithelial cells can divide their basal process before anaphase
- Source :
- The EMBO Journal. 27:3151-3163
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- Neuroepithelial (NE) cells, the primary stem and progenitor cells of the vertebrate central nervous system, are highly polarized and elongated. They retain a basal process extending to the basal lamina, while undergoing mitosis at the apical side of the ventricular zone. By studying NE cells in the embryonic mouse, chick and zebrafish central nervous system using confocal microscopy, electron microscopy and time-lapse imaging, we show here that the basal process of these cells can split during M phase. Splitting occurred in the basal-to-apical direction and was followed by inheritance of the processes by either one or both daughter cells. A cluster of anillin, an essential component of the cytokinesis machinery, appeared at the distal end of the basal process in prophase and was found to colocalize with F-actin at bifurcation sites, in both proliferative and neurogenic NE cells. GFP-anillin in the basal process moved apically to the cell body prior to anaphase onset, followed by basal-to-apical ingression of the cleavage furrow in telophase. The splitting of the basal process of M-phase NE cells has implications for cleavage plane orientation and the relationship between mitosis and cytokinesis.
- Subjects :
- Cytoplasm
Recombinant Fusion Proteins
Green Fluorescent Proteins
Neuroepithelial Cells
Ingression
Biology
Article
General Biochemistry, Genetics and Molecular Biology
Mice
Contractile Proteins
Genes, Reporter
medicine
Animals
Cleavage furrow
Telophase
Molecular Biology
Mitosis
Cells, Cultured
Zebrafish
Cytokinesis
Anaphase
Microscopy, Confocal
Microscopy, Video
General Immunology and Microbiology
General Neuroscience
Actins
Cell biology
Neuroepithelial cell
Microscopy, Electron
medicine.anatomical_structure
Basal lamina
Chickens
Cell Division
Subjects
Details
- ISSN :
- 14602075 and 02614189
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....aaa9b7e1211ffc61ee2addc6684f45d7