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RNA binding protein GNL3 up-regulates IL24 and PTN to promote the development of osteoarthritis
- Source :
- Life sciences. 267
- Publication Year :
- 2020
-
Abstract
- Osteoarthritis (OA) is a degenerative disease, which has a high incidence in middle-aged and elderly people and tends to occur in weight-bearing or active joints. Current treatment can only relieve symptoms and delay the progression of OA in result of its indistinct pathogenesis. In recent years, more and more studies have focused on the pathogenesis of OA. Nucleolar GTP binding protein 3 (GNL3) is associated with chondrogenic differentiation and can participate in genomic regulation as RNA binding protein (RBP). We used RNA sequencing (RNA-seq) to analyze the overall transcription level of the human cervical cancer cell line HeLa after GNL3 deletion. The results showed that downstream genes IL24 and PTN were down-regulated. IL24 takes part in the progression of OA by inducing articular osteocyte apoptosis, while PTN conducts to the progression of OA by promoting angiogenesis. We validated the results in the human chondrosarcoma cell line SW1353 and OA patients. Compared with the control group, GNL3, IL24 and PTN genes were elevated in OA specimens. This study explored the relationship between GNL3 and these two downstream genes, hoping to find biomarkers in the pathogenesis of osteoarthritis that can be used as therapeutic targets in the future.
- Subjects :
- 0301 basic medicine
Adult
Cartilage, Articular
Male
Transcriptional Activation
Angiogenesis
RNA-binding protein
030226 pharmacology & pharmacy
General Biochemistry, Genetics and Molecular Biology
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Chondrocytes
Transcription (biology)
GTP-Binding Proteins
Osteoarthritis
medicine
Humans
General Pharmacology, Toxicology and Pharmaceutics
Gene
Aged
business.industry
Interleukins
RNA
Nuclear Proteins
RNA-Binding Proteins
Cell Differentiation
Femur Head
General Medicine
Middle Aged
030104 developmental biology
medicine.anatomical_structure
GNL3
Osteocyte
Cancer research
Cytokines
Female
business
Carrier Proteins
Chondrogenesis
HeLa Cells
Transcription Factors
Subjects
Details
- ISSN :
- 18790631
- Volume :
- 267
- Database :
- OpenAIRE
- Journal :
- Life sciences
- Accession number :
- edsair.doi.dedup.....aaba23d3a87a029ba564c4c7197954fd