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miR-92a promotes cervical cancer cell proliferation, invasion, and migration by directly targeting PIK3R1
- Source :
- Journal of Clinical Laboratory Analysis
- Publication Year :
- 2021
-
Abstract
- Objective To clarify the role of miR‐92a in regulating the malignant progression of cervical cancer and its specific molecular mechanism. Methods qRT‐PCR was used to detect the differential expression of miR‐92a in cervical cancer and adjacent tissues. The effects of overexpression of miR‐92a on the proliferation, migration, and invasion of HeLa and SiHa cells were tested. Luciferase assays and rescue experiments were used to investigate the regulatory mechanism of miR‐92a on its downstream gene PIK3R1 and their interaction in the progression of cervical cancer. Results miR‐92a was significantly up‐regulated in cervical cancer tissues. Overexpression of miR‐92a significantly increased the ability of cervical cancer cells to proliferate, migrate, and invade. PIK3R1 was identified as a downstream gene of miR‐92a. In cervical cancer tissues, PIK3R1 was found to be down‐regulated and negatively correlated with the level of miR‐92a. Overexpression of PIK3R1 reversed the promotional effect of overexpressed miR‐92a on the proliferation, migration, and invasion of cervical cancer. Conclusion miR‐92a is up‐regulated in cervical cancer tissues. miR‐92a promotes the malignant development of cervical cancer by negatively regulating PIK3R1.<br />miR‐92a increases expression in cervical cancer tissues and promotes cervical cancer cell proliferation, invasion, and migration.
- Subjects :
- Microbiology (medical)
cervical cancer
Clinical Biochemistry
Uterine Cervical Neoplasms
HeLa
PIK3R1
Cell Movement
Cell Line, Tumor
Cervical carcinoma
medicine
Immunology and Allergy
Humans
Luciferase
Gene
Research Articles
Cell Proliferation
Cervical cancer
biology
Biochemistry (medical)
Public Health, Environmental and Occupational Health
Invasion and migration
Hematology
medicine.disease
biology.organism_classification
Up-Regulation
Class Ia Phosphatidylinositol 3-Kinase
Gene Expression Regulation, Neoplastic
Medical Laboratory Technology
MicroRNAs
Cancer research
Molecular mechanism
miR‐92a
Female
Research Article
Subjects
Details
- ISSN :
- 10982825
- Volume :
- 35
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of clinical laboratory analysis
- Accession number :
- edsair.doi.dedup.....aae1be4f70990b38d0f6eeed8b451366