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Cutting Edge: Characterization of Human Tissue-Resident Memory T Cells at Different Infection Sites in Patients with Tuberculosis
- Source :
- Journal of immunology (Baltimore, Md. : 1950). 204(9)
- Publication Year :
- 2019
-
Abstract
- Tissue-resident memory T cells (TRMs) have a key role in mediating the host defense against tuberculosis (TB) in mice, but their human counterparts have not been well characterized. In this article, we recruited patients with TB and determined TRM frequency, trafficking, activation marker expression, and cytokine production by flow or mass cytometry at different infection sites, including peripheral blood, pleural fluid, bronchoalveolar lavage fluid, and lung. We found a high frequency of TRMs at all infection sites apart from the peripheral blood. These TRMs exhibited a memory phenotype, were highly activated (based on CD38 and HLA-DR expression), and expressed high levels of trafficking (CCR5 and CXCR6) and exhaustion (PD-1) markers. When stimulated with Mycobacterium tuberculosis, TRMs secreted cytokines, including IFN-γ, TNF-α, and IL-2, and exhibited a multifunctional phenotype. TRMs limited intracellular M. tuberculosis replication in macrophages. These data inform our current understanding of immunosurveillance at different infection sites in patients with TB.
- Subjects :
- Adult
Male
Tuberculosis
medicine.medical_treatment
T-Lymphocytes
Immunology
Biology
CD38
Mycobacterium tuberculosis
Interferon-gamma
medicine
Immunology and Allergy
Humans
Mass cytometry
Lung
Cells, Cultured
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
Macrophages
biology.organism_classification
medicine.disease
Phenotype
Immunosurveillance
Bronchoalveolar lavage
Cytokine
Interleukin-2
Female
Cutting Edge
Bronchoalveolar Lavage Fluid
Immunologic Memory
Biomarkers
Subjects
Details
- ISSN :
- 15506606
- Volume :
- 204
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Accession number :
- edsair.doi.dedup.....aaea6dff5a3c03fd52f30d3505981a84