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The Transcriptional Co-Repressor C-Terminal Binding Protein (CtBP) Associates with Centrosomes During Mitosis
- Source :
- Cell Cycle. 5:530-537
- Publication Year :
- 2006
- Publisher :
- Informa UK Limited, 2006.
-
Abstract
- CtBP is a corepressor of transcription that acts by inhibiting coactivators and recruiting to promoter control elements (via interactions with DNA-binding transcription factors) a complex of proteins that modify histones, repress transcription and silence gene expression. There are two highly homologous genes, CtBP1 and CtBP2 that encode CtBP. In addition, a CtBP1-related protein has been described that has 11 fewer amino acids at its N-terminus than CtBP1. This variant of CtBP1--known as CtBP3 or BARS [Brefeldin A (BFA) ribosylated substrate]--plays a critical role in the fragmentation of the Golgi complex at the onset of mitosis. Although there are some reports of CtBP with a cytoplasmic distribution after transfection, it is unclear how in normal cells a nuclear corepressor regulates the behavior of the Golgi complex at the onset of mitosis. Using polyclonal rabbit antibodies that were raised against a human CtBP1-GST fusion-protein, here we show by immunofluorescence laser-scanning confocal microscopy that in mitotic cells a species of CtBP becomes associated with the centrosomes at the onset of prophase and then throughout mitosis. The association can be seen in both cycling mitotic cells and nocodazole-arrested cells. The interaction was confirmed by coimmunoprecipitation and centrosome isolation. Since centrosomes are considered to be the organising centre for Golgi morphogenesis, the interaction demonstrated here may explain how a nuclear corepressor of transcription can exert a regulatory effect on the Golgi complex at a specific stage of the cell cycle.
- Subjects :
- Transcription, Genetic
Recombinant Fusion Proteins
Mitosis
Cell Cycle Proteins
Protein Serine-Threonine Kinases
Biology
symbols.namesake
chemistry.chemical_compound
Prophase
Tubulin
Proto-Oncogene Proteins
Humans
Immunoprecipitation
Interphase
Molecular Biology
Transcription factor
Cells, Cultured
Cell Nucleus
Centrosome
Nocodazole
Reproducibility of Results
Cell Biology
Cell cycle
Golgi apparatus
Brefeldin A
Phosphoproteins
Molecular biology
DNA-Binding Proteins
Repressor Proteins
Alcohol Oxidoreductases
Protein Transport
chemistry
symbols
Corepressor
Developmental Biology
Subjects
Details
- ISSN :
- 15514005 and 15384101
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Cell Cycle
- Accession number :
- edsair.doi.dedup.....aaefd9f77e85ee03e44a0ea424c0d18a