Back to Search
Start Over
Keratinocytes Store the Antimicrobial Peptide Cathelicidin in Lamellar Bodies
- Source :
- Journal of Investigative Dermatology. 124:394-400
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- Innate immune defense against microbial pathogens occurs by physical barriers, by recruitment of cells such as neutrophils, NK cells, and macrophages, and by secretion of molecules with antimicrobial activity. Such molecules are produced by various epithelia including skin. The importance of antimicrobial peptides has been shown in cathelicidin-deficient mice, which have increased susceptibility to skin infection by Streptococcus. Although keratinocytes increase cathelicidin expression upon injury, their role relative to neutrophil cathelicidin and their sites of peptide storage and activation have not been elucidated. Herein, it is reported that cathelicidin predominantly resides in granules of the superficial epidermis and partially localizes in lamellar bodies as determined by immunogold electron microscopy and immunoblot of lamellar bodies isolated from mice. In cultured keratinocytes, cathelicidin displays a granular distribution and partially localizes within the Golgi apparatus. Cathelicidin processing can be observed by western blot analysis in keratinocyte extracts but not in conditioned media. Further, fluorescent bacteria colocalize with cathelicidin in granules both intracellularly and at the cell surface. These observations illustrate the immune defense potential of keratinocytes acting directly through storage and processing of antimicrobial peptides.
- Subjects :
- Keratinocytes
medicine.medical_treatment
Antimicrobial peptides
Golgi Apparatus
Dermatology
Biology
Lamellar granule
Cytoplasmic Granules
Biochemistry
Cathelicidin
Microbiology
antimicrobial peptides
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
Cathelicidins
cathelicidin
Escherichia coli
medicine
Humans
Secretion
lamellar bodies
Protein Precursors
innate immunity
Molecular Biology
Cells, Cultured
Escherichia coli Infections
030304 developmental biology
0303 health sciences
Innate immune system
Epidermis (botany)
Cell Biology
Immunogold labelling
3. Good health
Cell biology
Microscopy, Electron
medicine.anatomical_structure
Epidermal Cells
Microscopy, Fluorescence
lipids (amino acids, peptides, and proteins)
Keratinocyte
Antimicrobial Cationic Peptides
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 124
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Dermatology
- Accession number :
- edsair.doi.dedup.....ab26d14b4ce0e8a11779fa13daf69e16
- Full Text :
- https://doi.org/10.1111/j.0022-202x.2004.23443.x