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PrimeStore MTM and OMNIgene Sputum for the Preservation of Sputum for Xpert MTB/RIF Testing in Nigeria
- Source :
- Journal of Clinical Medicine, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Volume 8, Issue 12, r-IGTP: Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, Institut de Recerca Germans Trias i Pujol (IGTP)
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- Background: Xpert MTB/RIF (GX) for tuberculosis (TB) diagnosis is often located in reference laboratories, and sputum needs to be transported using a cold chain. Transport media to preserve sputum are available, but performance data under programmatic conditions are limited. Methods: Sputum samples were collected from patients with presumptive TB in Nigeria. One sputum was transported in a cold chain, tested immediately with GX and cultured. One sputum was swabbed and stored in PrimeStore-Molecular-Transport-Medium (Primestore), and the remainder was stored in OMNIGene-sputum (Omnigene), kept for seven days and tested with GX. Results: Of 248 patients, 63 were fresh-sputum culture-positive and 56 GX-positive (sensitivity 88.9%, 95% CI: 78.4&ndash<br />95.4%). Four of 185 culture-negative patients were GX-positive (specificity 97.8%, 94.6&ndash<br />99.4%). Omnigene GX and Primestore GX were positive in 56/62 (90.3%, 80.1&ndash<br />96.4%) and 49/62 (79.0%, 66.8&ndash<br />88.3%) culture-positive, respectively, and 1/185 (99.5%, 97.0&ndash<br />100.0%) and 3/185 (98.4%, 95.3&ndash<br />99.7%) were culture-negative patients. 14 Human Immunodeficiency Virus (HIV)-infected and 44 HIV-uninfected patients were culture-positive. Omnigene and Primestore detected 12/14 (85.7%, 57.2&ndash<br />98.2%) and 5/14 (35.7%, 12.8&ndash<br />64.9%) HIV-infected and 41/44 (93.2%, 81.3&ndash<br />98.6%) HIV-uninfected culture-positive patients. Interpretation: Omnigene stored and fresh sputum samples had similar GX results. The GX results of Primestore-stored samples were similar to those found in the fresh sputum of non-HIV infected patients, but GX-positivity was lower in HIV-infected patients. This was likely due to the lower amount of bacilli collected by the swab and transferred to PrimeStore.
- Subjects :
- 0301 basic medicine
qy_250
medicine.medical_specialty
Tuberculosis
diagnosis
030106 microbiology
Xpert MTB/RIF
Human immunodeficiency virus (HIV)
NDAS
wa_395
medicine.disease_cause
Gastroenterology
Article
qy_25
03 medical and health sciences
0302 clinical medicine
fluids and secretions
SDG 3 - Good Health and Well-being
RA0421
Internal medicine
RA0421 Public health. Hygiene. Preventive Medicine
parasitic diseases
medicine
030212 general & internal medicine
business.industry
OMNIGene-sputum
PrimeStore
General Medicine
medicine.disease
respiratory tract diseases
tuberculosis
Sputum
wf_220
wf_200
medicine.symptom
business
Subjects
Details
- ISSN :
- 20770383
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Medicine, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Volume 8, Issue 12, r-IGTP: Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, Institut de Recerca Germans Trias i Pujol (IGTP)
- Accession number :
- edsair.doi.dedup.....ab2c7422bbcd304a6a88d8d285b94ac9