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A handcuff model for the cohesin complex

Authors :
Kaiyi Li
Nenggang Zhang
Sergey G. Kuznetsov
Shyam K. Sharan
Pulivarthi H. Rao
Debananda Pati
Source :
The Journal of Cell Biology
Publication Year :
2008
Publisher :
Rockefeller University Press, 2008.

Abstract

The cohesin complex is responsible for the accurate separation of sister chromatids into two daughter cells. Several models for the cohesin complex have been proposed, but the one-ring embrace model currently predominates the field. However, the static configuration of the embrace model is not flexible enough for cohesins to perform their functions during DNA replication, transcription, and DNA repair. We used coimmunoprecipitation, a protein fragment complement assay, and a yeast two-hybrid assay to analyze the protein–protein interactions among cohesin subunits. The results show that three of the four human cohesin core subunits (Smc1, Smc3, and Rad21) interact with themselves in an Scc3 (SA1/SA2)-dependent manner. These data support a two-ring handcuff model for the cohesin complex, which is flexible enough to establish and maintain sister chromatid cohesion as well as ensure the fidelity of chromosome segregation in higher eukaryotes.

Details

ISSN :
15408140 and 00219525
Volume :
183
Database :
OpenAIRE
Journal :
Journal of Cell Biology
Accession number :
edsair.doi.dedup.....ab3c46b29cf2ae3e6dbbf3db2cfe18ee
Full Text :
https://doi.org/10.1083/jcb.200801157