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Analysis of accumulation patterns and preliminary study on the condensation mechanism of proanthocyanidins in the tea plant [Camellia sinensis]
- Source :
- Scientific Reports
- Publication Year :
- 2015
- Publisher :
- Springer Science and Business Media LLC, 2015.
-
Abstract
- In the present study, proanthocyanidins were qualitatively and quantitatively identified using hydrolysis and thiolysis assays, NP-HPLC, HPLC-ESI-MS, MALDI-TOF-MS, 1H-NMR and 13C-NMR techniques in different organs of tea plants. The results showed that in leaves, the tri-hydroxyl, cis- and galloylated flavan-3-ols were the main monomeric catechins units and (epi)catechin was found to be the major unit of polymeric flavan-3-ols when the degree of polymerization was greater than five. In roots, the PAs were found to be abundant and epicatechin formed the predominant extension unit of oligomeric and polymeric PAs. In order to understand the mechanism of proanthocyanidins polymerization, auto-condensation of the flavan-3-ols was investigated. The results showed that the same trimers (m/z 865) were detected in the extracts of tea plants and in the non-enzymatic in vitro assay, in weak acid as well as weak alkaline solutions at room temperature, when the substrates used were either procyanidin B2 and monomeric flavan-3-ols (epicatechin or catechin), or only procyanidin B2. This suggested that procyanidin B2 not only released carbocation as electrophilic upper units, but also could be used as nucleophilic lower units directly itself, to form the procyanidin trimer in vitro or in vivo.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Magnetic Resonance Spectroscopy
Degree of polymerization
Plant Roots
Article
Camellia sinensis
Catechin
Polymerization
chemistry.chemical_compound
Hydrolysis
Biflavonoids
Organic chemistry
Proanthocyanidins
Procyanidin B2
Chromatography, High Pressure Liquid
Multidisciplinary
Molecular Structure
Biosynthetic Pathways
Plant Leaves
Monomer
chemistry
Biochemistry
Thiolysis
Proanthocyanidin
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Dimerization
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....ab5d684069b9233e29a8d64f87980602