Back to Search
Start Over
The direct targets of CBFs: In cold stress response and beyond
- Source :
- Journal of integrative plant biologyREFERENCES. 63(11)
- Publication Year :
- 2021
-
Abstract
- Cold acclimation in Arabidopsis thaliana triggers a significant transcriptional reprogramming altering the expression patterns of thousands of cold-responsive (COR) genes. Essential to this process is the C-repeat binding factor (CBF)-dependent pathway, involving the activity of AP2/ERF (APETALA2/ethylene-responsive factor)-type CBF transcription factors required for plant cold acclimation. In this study, we performed chromatin immunoprecipitation assays followed by deep sequencing (ChIP-seq) to determine the genome-wide binding sites of the CBF transcription factors. Cold-induced CBF proteins specifically bind to the conserved C-repeat (CRT)/dehydration-responsive elements (CRT/DRE; G/ACCGAC) of their target genes. A Gene Ontology enrichment analysis showed that 1,012 genes are targeted by all three CBFs. Combined with a transcriptional analysis of the cbf1,2,3 triple mutant, we define 146 CBF regulons as direct CBF targets. In addition, the CBF-target genes are significantly enriched in functions associated with hormone, light, and circadian rhythm signaling, suggesting that the CBFs act as key integrators of endogenous and external environmental cues. Our findings not only define the genome-wide binding patterns of the CBFs during the early cold response, but also provide insights into the role of the CBFs in regulating multiple biological processes of plants.
- Subjects :
- Arabidopsis
Plant Science
Biochemistry
Regulon
General Biochemistry, Genetics and Molecular Biology
Deep sequencing
Plant Growth Regulators
Gene Expression Regulation, Plant
Cold acclimation
Arabidopsis thaliana
Transcription factor
Gene
biology
Arabidopsis Proteins
Cold-Shock Response
biology.organism_classification
Lipid Metabolism
Cell biology
Trans-Activators
Carbohydrate Metabolism
Chromatin Immunoprecipitation Sequencing
Chromatin immunoprecipitation
circulatory and respiratory physiology
Transcription Factors
Subjects
Details
- ISSN :
- 17447909
- Volume :
- 63
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of integrative plant biologyREFERENCES
- Accession number :
- edsair.doi.dedup.....ab63bb1f43aa58b0e166046dce3f535a