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Role of Vascular Endothelial Cells in Disseminated Intravascular Coagulation Induced by Seawater Immersion in a Rat Trauma Model
- Source :
- BioMed Research International, BioMed Research International, Vol 2017 (2017)
- Publication Year :
- 2017
- Publisher :
- Hindawi Limited, 2017.
-
Abstract
- Trauma complicated by seawater immersion is a complex pathophysiological process with higher mortality than trauma occurring on land. This study investigated the role of vascular endothelial cells (VECs) in trauma development in a seawater environment. An open abdominal injury rat model was used. The rat core temperatures in the seawater (SW, 22°C) group and normal sodium (NS, 22°C) group declined equivalently. No rats died within 12 hours in the control and NS groups. However, the median lethal time of the rats in the SW group was only 260 minutes. Among the 84 genes involved in rat VEC biology, the genes exhibiting the high expression changes (84.62%, 11/13) on a qPCR array were associated with thrombin activity. The plasma activated partial thromboplastin time and fibrinogen and vWF levels decreased, whereas the prothrombin time and TFPI levels increased, indicating intrinsic and extrinsic coagulation pathway activation and inhibition, respectively. The plasma plasminogen, FDP, and D-dimer levels were elevated after 2 hours, and those of uPA, tPA, and PAI-1 exhibited marked changes, indicating disseminated intravascular coagulation (DIC). Additionally, multiorgan haemorrhagia was observed. It indicated that seawater immersion during trauma may increase DIC, elevating mortality. VECs injury might play an essential role in this process.
- Subjects :
- Male
0301 basic medicine
Pathology
medicine.medical_specialty
Time Factors
Article Subject
lcsh:Medicine
Abdominal Injuries
030204 cardiovascular system & hematology
Fibrinogen
General Biochemistry, Genetics and Molecular Biology
Andrology
03 medical and health sciences
0302 clinical medicine
Immersion
medicine
Animals
Seawater
Rats, Wistar
Blood Coagulation
Disseminated intravascular coagulation
Prothrombin time
Regulation of gene expression
General Immunology and Microbiology
medicine.diagnostic_test
business.industry
Gene Expression Profiling
lcsh:R
Endothelial Cells
General Medicine
Disseminated Intravascular Coagulation
medicine.disease
Survival Analysis
Pathophysiology
Disease Models, Animal
030104 developmental biology
Gene Expression Regulation
Wounds and Injuries
Normal sodium
business
Research Article
medicine.drug
Partial thromboplastin time
Subjects
Details
- ISSN :
- 23146141 and 23146133
- Volume :
- 2017
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....ab7aedda2f4e8786456ae246d3556402