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Down-Regulation of miR-129-5p Inhibits Growth and Induces Apoptosis in Laryngeal Squamous Cell Carcinoma by Targeting APC
- Source :
- PLoS ONE, PLoS ONE, Vol 8, Iss 10, p e77829 (2013)
- Publication Year :
- 2013
- Publisher :
- Public Library of Science, 2013.
-
Abstract
- miRNAs regulate gene expression and are key mediators of tumourigenesis. miR-129 has diverse effects in tumours, but its role in laryngeal squamous cell carcinoma (LSCC) remains unknown. This article focuses on the role of miR-129-5p in LSCC. We show miR-129-5p is upregulated in primary LSCC tumours and correlated with advanced disease. Down-regulating miR-129-5p suppressed cell proliferation and migration, and caused cell cycle arrest in Hep-2 cell lines. Downregulation of miR-129-5p alone is sufficient to induce apoptosis both in vivo and in vitro. Moreover, the growth of LSCC xenograft exposed to miR-129-5p antisense oligonucleotides (ASO) in BALB/c mice was markedly inhibited. In addition, we found that miR-129-5p targeted adenomatous polyposis coli (APC) to release inhibition of Wnt signalling causing cell growth and tumourigenesis. Our results suggest miR-129-5p functions as an oncogene in LSCC by repressing APC and is a potential therapeutic target for LSCC.
- Subjects :
- Cell cycle checkpoint
Adenomatous polyposis coli
Adenomatous Polyposis Coli Protein
lcsh:Medicine
Apoptosis
Mice
Downregulation and upregulation
Cell Movement
microRNA
Animals
lcsh:Science
Laryngeal Neoplasms
Cell Proliferation
Mice, Inbred BALB C
Multidisciplinary
biology
Oncogene
Cell growth
lcsh:R
Oligonucleotides, Antisense
Molecular biology
Gene Expression Regulation, Neoplastic
MicroRNAs
Cell culture
biology.protein
Cancer research
Carcinoma, Squamous Cell
lcsh:Q
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....ab819229d388e60f112910cbc41ef7a8