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The effect of two ribonucleases on the production of Shiga toxin and stx-bearing bacteriophages in Enterohaemorrhagic Escherichia coli
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Enterohaemorrhagic Escherichia coli (EHEC) comprise a group of intestinal pathogens responsible for a range of illnesses, including kidney failure and neurological compromise. EHEC produce critical virulence factors, Shiga toxin (Stx) 1 or 2, and the synthesis of Stx2 is associated with worse disease manifestations. Infected patients only receive supportive treatment because some conventional antibiotics enable toxin production. Shiga toxin 2 genes (stx2) are carried in λ-like bacteriophages (stx2-phages) inserted into the EHEC genome as prophages. Factors that cause DNA damage induce the lytic cycle of stx2-phages, leading to Stx2 production. The phage Q protein is critical for transcription antitermination of stx2 and phage lytic genes. This study reports that deficiency of two endoribonucleases (RNases), E and G, significantly delayed cell lysis and impaired production of both Stx2 and stx2-phages, unlike deficiency of either enzyme alone. Moreover, scarcity of both enzymes reduced the concentrations of Q and stx2 transcripts and slowed cell growth.
- Subjects :
- Virulence Factors
Mitomycin
Science
Virulence
Viral Plaque Assay
medicine.disease_cause
Shiga Toxin 1
Shiga Toxin 2
Microbiology
Article
Shiga Toxin
Ribonucleases
fluids and secretions
STX2
hemic and lymphatic diseases
medicine
Bacteriophages
Gene
Prophage
Escherichia coli Infections
Multidisciplinary
biology
Toxin
Shiga toxin
Gene Expression Regulation, Bacterial
Bacterial pathogenesis
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Transcription antitermination
Lytic cycle
Enterohemorrhagic Escherichia coli
biology.protein
bacteria
Medicine
Plasmids
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....ab8f4f0e8c0a02ef0465605cae68257c