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A stable isotope dilution method for a highly accurate analysis of karrikins
- Source :
- Plant Methods, Vol 17, Iss 1, Pp 1-13 (2021), Plant Methods
- Publication Year :
- 2021
- Publisher :
- BMC, 2021.
-
Abstract
- Background Karrikins (KARs) are recently described group of plant growth regulators with stimulatory effects on seed germination, seedling growth and crop productivity. So far, an analytical method for the simultaneous targeted profiling of KARs in plant tissues has not been reported. Results We present a sensitive method for the determination of two highly biologically active karrikins (KAR1 and KAR2) in minute amounts of plant material (1 was employed as an internal standard for the validation of KAR quantification using a stable isotope dilution method. The application of the matrix-matched calibration series in combination with the internal standard method yields a high level of accuracy and precision in triplicate, on average bias 3.3% and 2.9% RSD, respectively. The applicability of this analytical approach was confirmed by the successful analysis of karrikins in Arabidopsis seedlings grown on media supplemented with different concentrations of KAR1 and KAR2 (0.1, 1.0 and 10.0 µmol/l). Conclusions Our results demonstrate the usage of methodology for routine analyses and for monitoring KARs in complex biological matrices. The proposed method will lead to better understanding of the roles of KARs in plant growth and development.
- Subjects :
- Plant growth
Accuracy and precision
Internal standard
Chromatography
Chemistry
Research
Extraction (chemistry)
Ultra-high performance liquid chromatography (UHPLC)
Plant Science
Smoke water
lcsh:Plant culture
Mass spectrometry
Stable isotope dilution
Tandem mass spectrometry (MS/MS)
lcsh:Biology (General)
Germination
Genetics
Calibration
Karrikins
lcsh:SB1-1110
Stable isotope dilution method
lcsh:QH301-705.5
Biotechnology
Stable isotope labelled standard
Subjects
Details
- Language :
- English
- ISSN :
- 17464811
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Plant Methods
- Accession number :
- edsair.doi.dedup.....aba110b109ed172c034dfb5890b45a29