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Prolongation of Cardiac Allograft Survival by Endometrial Regenerative Cells: Focusing on B-Cell Responses
- Source :
- Stem Cells Translational Medicine
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- Endometrial regenerative cells (ERCs) have been recently evaluated as an attractive candidate source for emerging stem cell therapies in immunosuppression, but their role in immunoregulation is not fully understood. The present study was designed to investigate their effects, especially on B-cell responses in heart transplantation. In this study, ERCs were noninvasively obtained from menstrual blood. Heart transplantation was performed between C57BL/6 (H-2b) donor mice and BALB/c (H-2d) recipients. B-cell activation and antibody levels were determined using fluorescence-activated cell sorting, enzyme-linked immunosorbent assay and ELISpot. In this study, we demonstrated that ERCs negatively regulated B-cell maturation and activation in vitro without affecting their viability. ERC treatment prolonged cardiac allograft survival in mice, which was correlated with a decrease in IgM and IgG deposition and circulating antidonor antibodies, as well as with reduction in frequencies of antidonor antibody-secreting CD19+ B cells. In addition, upon ex vivo stimulation, B cells from ERC-treated heart transplant recipients had impaired proliferation capacity and produced less IgM and IgG antibody. Moreover, ERC treatment of mice receiving ovalbumin (OVA)-aluminum hydroxide vaccine resulted in significant lower numbers of anti-OVA IgG antibody-secreting splenic B cells and lower anti-OVA antibody titres. Our results indicate that therapeutic effects of ERCs may be attributed at least in part by their B-cell suppression and humoral response inhibition, suggesting the potential use of ERCs for attenuating antibody-mediated allograft rejection.
- Subjects :
- Lipopolysaccharides
0301 basic medicine
medicine.medical_treatment
Heart transplantation
030230 surgery
Endometrial regenerative cells
Endometrium
Mice
0302 clinical medicine
Translational Research Articles and Reviews
Medicine
B-Lymphocytes
Mice, Inbred BALB C
biology
ELISPOT
Graft Survival
Vaccination
Cell Differentiation
Immunosuppression
General Medicine
Allografts
medicine.anatomical_structure
Female
Stem cell
Antibody
Cell Survival
Ovalbumin
Antibodies
CD19
03 medical and health sciences
Animals
Regeneration
B cell
Cell Proliferation
Antibody‐mediated rejection
business.industry
Cell Biology
Mice, Inbred C57BL
030104 developmental biology
Immunoglobulin M
Immunoglobulin G
Immunology
biology.protein
business
Biomarkers
Ex vivo
Tissue‐Specific Progenitor and Stem Cells
B lymphocytes
Developmental Biology
Subjects
Details
- ISSN :
- 21576580 and 21576564
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Stem Cells Translational Medicine
- Accession number :
- edsair.doi.dedup.....abf958efec895ef76e02fb3e1aaedb7d