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Archaeal communities of Arctic methane-containing permafrost
- Source :
- FEMS Microbiology Ecology. 92:fiw135
- Publication Year :
- 2016
- Publisher :
- Oxford University Press (OUP), 2016.
-
Abstract
- In the present study, we used culture-independent methods to investigate the diversity of methanogenic archaea and their distribution in five permafrost samples collected from a borehole in the Kolyma River Lowland (north-east of Russia). Total DNA was extracted from methane-containing permafrost samples of different age and amplified by PCR. The resulting DNA fragments were cloned. Phylogenetic analysis of the sequences showed the presence of archaea in all studied samples; 60%-95% of sequences belonged to the Euryarchaeota. Methanogenic archaea were novel representatives of Methanosarcinales, Methanomicrobiales, Methanobacteriales and Methanocellales orders. Bathyarchaeota (Miscellaneous Crenarchaeota Group) representatives were found among nonmethanogenic archaea in all the samples studied. The Thaumarchaeota representatives were not found in the upper sample, whereas Woesearchaeota (formerly DHVEG-6) were found in the three deepest samples. Unexpectedly, the greatest diversity of archaea was observed at a depth of 22.3 m, probably due to the availability of the labile organic carbon and/or due to the migration of the microbial cells during the freezing front towards the bottom.
- Subjects :
- 0301 basic medicine
Thaumarchaeota
Methanogenesis
030106 microbiology
Permafrost
Euryarchaeota
Applied Microbiology and Biotechnology
Microbiology
Methanomicrobiales
Russia
03 medical and health sciences
Crenarchaeota
RNA, Ribosomal, 16S
Phylogeny
Soil Microbiology
Ecology
biology
Arctic Regions
Methanosarcinales
biology.organism_classification
Archaea
DNA, Archaeal
Methanobacteriales
Methane
Subjects
Details
- ISSN :
- 15746941
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Ecology
- Accession number :
- edsair.doi.dedup.....abf9e7feb64c95af5154240b7545d1f0