Back to Search
Start Over
Genome-wide Mapping of HATs and HDACs Reveals Distinct Functions in Active and Inactive Genes
- Source :
- Cell. (5):1019-1031
- Publisher :
- Elsevier Inc.
-
Abstract
- SummaryHistone acetyltransferases (HATs) and deacetylases (HDACs) function antagonistically to control histone acetylation. As acetylation is a histone mark for active transcription, HATs have been associated with active and HDACs with inactive genes. We describe here genome-wide mapping of HATs and HDACs binding on chromatin and find that both are found at active genes with acetylated histones. Our data provide evidence that HATs and HDACs are both targeted to transcribed regions of active genes by phosphorylated RNA Pol II. Furthermore, the majority of HDACs in the human genome function to reset chromatin by removing acetylation at active genes. Inactive genes that are primed by MLL-mediated histone H3K4 methylation are subject to a dynamic cycle of acetylation and deacetylation by transient HAT/HDAC binding, preventing Pol II from binding to these genes but poising them for future activation. Silent genes without any H3K4 methylation signal show no evidence of being bound by HDACs.
- Subjects :
- Gene Expression
Methylation
environment and public health
Histone Deacetylases
Article
General Biochemistry, Genetics and Molecular Biology
Cell Line
Histones
Histone H2A
Histone methylation
parasitic diseases
Humans
Histone code
Phosphorylation
Histone Acetyltransferases
Genetics
biology
Genome, Human
Biochemistry, Genetics and Molecular Biology(all)
Acetylation
DNA
Chromatin
Histone Deacetylase Inhibitors
Histone
Histone methyltransferase
biology.protein
RNA Polymerase II
Subjects
Details
- Language :
- English
- ISSN :
- 00928674
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Cell
- Accession number :
- edsair.doi.dedup.....ac0a656b5bc83b41f6443e5429d803d5
- Full Text :
- https://doi.org/10.1016/j.cell.2009.06.049