A rellable technique for large-scale DNA separation

The various DNA species were separated electrophoretically in preparative scale in agarose gel (for example, up to 10 mg of phage f1-infected Escherichia coli DNAs per run) using general column electrophoretic equipment; homogeneous DNA fractions were eluted from the DNA-containing gel slices with a special electroelution cell that is able to achieve the quantitative recovery of DNA. The resolved Col E1 DNAs may be specifically cleaved with EcoRI restriction enzyme without further purification. The described methods were applied for separation of DNA fragments obtained after the specific fragmentation of E. coli DNA with EcoRI endonuclease.