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Serum anti-EIF3A autoantibody as a potential diagnostic marker for hepatocellular carcinoma
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
- Publication Year :
- 2018
-
Abstract
- Tumor-associated autoantibodies are promising diagnostic biomarkers for early detection of tumors. We have screened a novel tumor-associated autoantibody in hepatocellular carcinoma (HCC) model mice. Its target antigen was identified as eukaryotic translation initiation factor 3 subunit A (EIF3A) by proteomic analysis, and the elevated expression of EIF3A in HCC tissues of tumor model mice as well as human patients was shown. Also, its existence in tumor-derived exosomes was revealed, which seem to be the cause of tumor-associated autoantibody production. To use serum anti-EIF3A autoantibody as biomarker, ELISA detecting anti-EIF3A autoantibody in human serum was performed using autoantibody-specific epitope. For the sensitive detection of serum autoantibodies its specific conformational epitopes were screened from the random cyclic peptide library, and a streptavidin antigen displaying anti-EIF3A autoantibody-specific epitope, XC90p2(-CPVRSGFPC-), was used as capture antigen. It distinguished patients with HCC (n = 102) from healthy controls (n = 0285) with a sensitivity of 79.4% and specificity of 83.5% (AUC = 0.87). Also, by simultaneously detecting with other HCC biomarkers, including alpha-fetoprotein, HCC diagnostic sensitivity improved from 79.4% to 85%. Collectively, we suggest that serum anti-EIF3A autoantibody is a useful biomarker for the diagnosis of HCC and the combinational detection of related biomarkers can enhance the accuracy of the cancer diagnosis.
- Subjects :
- 0301 basic medicine
Adult
Male
Carcinoma, Hepatocellular
Hepatocellular carcinoma
Eukaryotic Initiation Factor-3
lcsh:Medicine
Mice, Transgenic
Epitope
Article
03 medical and health sciences
Mice
0302 clinical medicine
Antigen
medicine
Biomarkers, Tumor
Initiation factor
Animals
Humans
lcsh:Science
Aged
Autoantibodies
Aged, 80 and over
Multidisciplinary
business.industry
lcsh:R
Liver Neoplasms
Autoantibody
Cancer
Diagnostic markers
Middle Aged
medicine.disease
Microvesicles
030104 developmental biology
Cancer research
Biomarker (medicine)
lcsh:Q
Female
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....ac3ccf608bde64929333dd044cd9b55d