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Short-Term Stability of Pathogen-Specific Nucleic Acid Targets in Clinical Samples
- Source :
- Journal of Clinical Microbiology. 50:4147-4150
- Publication Year :
- 2012
- Publisher :
- American Society for Microbiology, 2012.
-
Abstract
- The stability of pathogen-specific DNA or RNA amplification targets in clinical samples following short-term storage at room temperature, 4°C, and −80°C was assessed by real-time PCR. In purified nucleic acid extracts, both DNA and RNA targets were stable for up to 30 days, irrespective of storage temperature. In unextracted samples, temperature-dependent loss of targets ( P < 0.05) was observed in serum and cerebrospinal fluid specimens, while no changes were observed for EDTA blood samples.
- Subjects :
- Microbiology (medical)
Time Factors
Temperature
RNA
Bacteriology
Biology
Real-Time Polymerase Chain Reaction
Molecular biology
Specimen Handling
chemistry.chemical_compound
Cerebrospinal fluid
Real-time polymerase chain reaction
Molecular Diagnostic Techniques
Biochemistry
chemistry
Nucleic Acids
Short term stability
Nucleic acid
Humans
Pathogen
DNA
RNA amplification
Subjects
Details
- ISSN :
- 1098660X and 00951137
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....ac68861e353e9dff1918645adb7e812b