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Forskolin Protected against Streptozotocin-Induced Diabetic Cardiomyopathy via Inhibition of Oxidative Stress and Cardiac Fibrosis in Mice
- Source :
- BioMed Research International, BioMed Research International, Vol 2021 (2021)
- Publication Year :
- 2021
- Publisher :
- Hindawi Limited, 2021.
-
Abstract
- Background. Diabetic cardiomyopathy is one of the cardiac complications in diabetes patients, eventually resulting in heart failure and increasing morbidity and mortality. Oxidative stress is a critical pathological feature in diabetic hearts, contributing to the development of DCM. Forskolin (FSK) was shown to reduce oxidative stress. This study was aimed at investigating the effects of FSK on diabetic hearts and the relevant molecular mechanisms. Methods. Streptozotocin- (STZ-) induced diabetes in mice was treated with FSK through intraperitoneal injection. Cardiac functions were evaluated by echocardiography. Hematoxylin-eosin and Masson trichrome staining was employed to determine heart morphological changes and cardiac fibrosis, respectively. Cardiac fibrosis-related markers were detected by western blot. Superoxide dismutase activity, reduced/oxidized glutathione ratio, and malondialdehyde concentration in left ventricles were determined using respective commercial kits. Results. Abnormal cardiac diastolic dysfunction and cardiac fibrosis were observed in diabetic hearts. FSK treatment significantly improved the cardiac diastolic function and attenuated the abnormal morphological change in diabetic hearts. Moreover, FSK treatment in diabetic mice decreased the expression of fibronectin, collagen I, TGF-β, and α-SMA and reduced myocardial fibrosis. Furthermore, we observed that FSK significantly blocked oxidative stress in diabetic hearts. Conclusions. Our study demonstrates that FSK protects against the development of DCM in STZ-induced diabetes in mice. Our study suggests that FSK might be a potential target for drug development in treating DCM.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Article Subject
Diabetic Cardiomyopathies
Cardiac fibrosis
Diastole
Apoptosis
030204 cardiovascular system & hematology
medicine.disease_cause
General Biochemistry, Genetics and Molecular Biology
Diabetes Mellitus, Experimental
Mice
03 medical and health sciences
0302 clinical medicine
Transforming Growth Factor beta
Internal medicine
Diabetes mellitus
Diabetic cardiomyopathy
medicine
Animals
Humans
General Immunology and Microbiology
business.industry
Myocardium
Colforsin
Heart
General Medicine
medicine.disease
Streptozotocin
Fibrosis
Actins
Disease Models, Animal
Oxidative Stress
030104 developmental biology
Endocrinology
Gene Expression Regulation
Heart failure
cardiovascular system
Medicine
Myocardial fibrosis
business
Oxidative stress
Research Article
medicine.drug
Subjects
Details
- ISSN :
- 23146141 and 23146133
- Volume :
- 2021
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....ac6ac52c38cb544b574ffcfcfefe0b36