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Rhythmic control of AANAT translation by hnRNP Q in circadian melatonin production
- Source :
- Genes & Development. 21:797-810
- Publication Year :
- 2007
- Publisher :
- Cold Spring Harbor Laboratory, 2007.
-
Abstract
- The circadian rhythm of pineal melatonin requires the nocturnal increment of serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase [AANAT]) protein. To date, only limited information is available in the critical issue of how AANAT protein expression is up-regulated exclusively at night regardless of its species-specific mRNA profiles. Here we show that the circadian timing of AANAT protein expression is regulated by rhythmic translation of AANAT mRNA. This rhythmic control is mediated by both a highly conserved IRES (internal ribosome entry site) element within the AANAT 5′ untranslated region and its partner hnRNP Q (heterogeneous nuclear ribonucleoprotein Q) with a peak in the middle of the night. Consistent with the enhancing role of hnRNP Q in AANAT IRES activities, knockdown of the hnRNP Q level elicited a dramatic decrease of peak amplitude in the AANAT protein profile parallel to reduced melatonin production in pinealocytes. This translational regulation of AANAT mRNA provides a novel aspect for achieving the circadian rhythmicity of vertebrate melatonin.
- Subjects :
- medicine.medical_specialty
Heterogeneous nuclear ribonucleoprotein
AANAT
Molecular Sequence Data
Biology
Arylalkylamine N-Acetyltransferase
Models, Biological
Pineal Gland
Heterogeneous-Nuclear Ribonucleoproteins
Pinealocyte
Melatonin
Pineal gland
Internal medicine
Translational regulation
Genetics
medicine
Animals
Humans
RNA, Messenger
Circadian rhythm
Sheep
Base Sequence
Models, Genetic
General Neuroscience
General Medicine
Molecular biology
Circadian Rhythm
Rats
Cell biology
Internal ribosome entry site
Endocrinology
medicine.anatomical_structure
Protein Biosynthesis
Arylalkylamine
5' Untranslated Regions
Research Paper
Developmental Biology
medicine.drug
Subjects
Details
- ISSN :
- 15495477 and 08909369
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Genes & Development
- Accession number :
- edsair.doi.dedup.....ac9acbbefa2574aac8730855b40f3a01