Back to Search
Start Over
Regulation of transcription of nucleotide-binding leucine-rich repeat-encoding genes SNC1 and RPP4 via H3K4 trimethylation
- Source :
- Plant physiology. 162(3)
- Publication Year :
- 2013
-
Abstract
- Plant nucleotide-binding leucine-rich repeat (NB-LRR) proteins serve as intracellular sensors to detect pathogen effectors and trigger immune responses. Transcription of the NB-LRR-encoding Resistance (R) genes needs to be tightly controlled to avoid inappropriate defense activation. How the expression of the NB-LRR R genes is regulated is poorly understood. The Arabidopsis (Arabidopsis thaliana) suppressor of npr1-1, constitutive 1 (snc1) mutant carries a gain-of-function mutation in a Toll/Interleukin1 receptor-like (TIR)-NB-LRR-encoding gene, resulting in the constitutive activation of plant defense responses. A snc1 suppressor screen identified modifier of snc1,9 (mos9), which partially suppresses the autoimmune phenotypes of snc1. Positional cloning revealed that MOS9 encodes a plant-specific protein of unknown function. Expression analysis showed that MOS9 is required for the full expression of TIR-NB-LRR protein-encoding RECOGNITION OF PERONOSPORA PARASITICA 4 (RPP4) and SNC1, both of which reside in the RPP4 cluster. Coimmunoprecipitation and mass spectrometry analyses revealed that MOS9 associates with the Set1 class lysine 4 of histone 3 (H3K4) methyltransferase Arabidopsis Trithorax-Related7 (ATXR7). Like MOS9, ATXR7 is also required for the full expression of SNC1 and the autoimmune phenotypes in the snc1 mutant. In atxr7 mutant plants, the expression of RPP4 is similarly reduced, and resistance against Hyaloperonospora arabidopsidis Emwa1 is compromised. Consistent with the attenuated expression of SNC1 and RPP4, trimethylated H3K4 marks are reduced around the promoters of SNC1 and RPP4 in mos9 plants. Our data suggest that MOS9 functions together with ATXR7 to regulate the expression of SNC1 and RPP4 through H3K4 methylation, which plays an important role in fine-tuning their transcription levels and functions in plant defense.
- Subjects :
- Repetitive Sequences, Amino Acid
Positional cloning
Physiology
Mutant
Arabidopsis
Plant Science
Leucine-rich repeat
Leucine-Rich Repeat Proteins
Methylation
Histones
Transcription (biology)
Gene Expression Regulation, Plant
Genetics
Signaling and Response
Cloning, Molecular
Promoter Regions, Genetic
Regulation of gene expression
Hyaloperonospora arabidopsidis
biology
Arabidopsis Proteins
Lysine
fungi
Nuclear Proteins
Proteins
Promoter
Methyltransferases
biology.organism_classification
Plants, Genetically Modified
Oomycetes
Mutation
Subjects
Details
- ISSN :
- 15322548
- Volume :
- 162
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Plant physiology
- Accession number :
- edsair.doi.dedup.....acb86c633e0bbd881050ea2df7b8cf35