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Tongguanteng injection reverses paclitaxel resistance via upregulation of TAB1 expression in ovarian cancer in vitro and in vivo
- Source :
- Journal of Ethnopharmacology. 300:115728
- Publication Year :
- 2023
- Publisher :
- Elsevier BV, 2023.
-
Abstract
- Tongguanteng injection (TGT), the water extract from the stem of the Traditional Chinese hebal medicine of Marsdenia tenacissima (Roxb.) Wight et Arn. has been used as anticancer remedy for decades. TGT was not only used in the treatment of many malignant cancers extensively, but also an adjuvant anticancer drug with chemotherapeutics clinically.To evaluate the effects of TGT on reversing paclitaxel (PTX) resistance and investigate the potential mechanism related to TAB1 in ovarian cancer (OC) in vitro and in vivo.The synergistic effect and reversal ratio were determined by CCK8 assay and median-effect principle after the combination of TGT and PTX in OC A2780 and its PTX-resistant (A2780/T) cells. The biological functions in cell apoptosis, migration and invasion of A2780/T cells treated by PTX 4 μM with TGT 20, 40, 80 mg⋅mLTGT combined with PTX showed the synergistic effect (CI1), which could reverse the ICOur results revealed that Tongguanteng injection could reverse paclitaxel resistance and the potential mechanism might be associated with the activation of TAB1/TAK1/p38 MAPK signaling pathway in OC in vitro and in vivo. TAB1 might be a pivotal target for reversing PTX resistance. This study will provide a theoretical basis for the combination of Tongguanteng injection and paclitaxel in clinic.
- Subjects :
- Ovarian Neoplasms
Pharmacology
History
Paclitaxel
Polymers and Plastics
Mice, Nude
Antineoplastic Agents
Apoptosis
Antineoplastic Agents, Phytogenic
p38 Mitogen-Activated Protein Kinases
Industrial and Manufacturing Engineering
Up-Regulation
Mice
Proto-Oncogene Proteins c-bcl-2
Drug Resistance, Neoplasm
Cell Line, Tumor
Drug Discovery
Animals
Humans
Female
Business and International Management
Adaptor Proteins, Signal Transducing
bcl-2-Associated X Protein
Subjects
Details
- ISSN :
- 03788741
- Volume :
- 300
- Database :
- OpenAIRE
- Journal :
- Journal of Ethnopharmacology
- Accession number :
- edsair.doi.dedup.....accfe3bcd9d9100b176f2bb0f13dd0ae