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LEAFY activity is post-transcriptionally regulated by BLADE ON PETIOLE2 and CULLIN3 in Arabidopsis
- Source :
- New Phytologist, New Phytologist, Wiley, 2018, 220 (2), pp.579-592. ⟨10.1111/nph.15329⟩, New Phytologist, 2018, 220 (2), pp.579-592. ⟨10.1111/nph.15329⟩
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- International audience; The Arabidopsis LEAFY (LFY) transcription factor is a key regulator of floral meristem emergence and identity. LFY interacts genetically and physically with UNUSUAL FLORAL ORGANS, a substrate adaptor of CULLIN1-RING ubiquitin ligase complexes (CRL1). The functionally redundant genes BLADE ON PETIOLE1 (BOP1) and -2 (BOP2) are potential candidates to regulate LFY activity and have recently been shown to be substrate adaptors of CULLIN3 (CUL3)-RING ubiquitin ligases (CRL3). We tested the hypothesis that LFY activity is controlled by BOPs and CUL3s in plants and that LFY is a substrate for ubiquitination by BOP-containing CRL3 complexes. When constitutively expressed, LFY activity is fully dependent on BOP2 as well as on CUL3A and B to regulate target genes such as APETALA1 and to induce ectopic flower formation. We also show that LFY and BOP2 proteins interact physically and that LFY-dependent ubiquitinated species are produced in vitro in a reconstituted cell-free CRL3 system in the presence of LFY, BOP2 and CUL3. This new post-translational regulation of LFY activity by CRL3 complexes makes it a unique transcription factor subjected to a positive dual regulation by both CRL1 and CRL3 complexes and suggests a novel mechanism for promoting flower development.
- Subjects :
- 0301 basic medicine
Transcription, Genetic
Physiology
[SDV]Life Sciences [q-bio]
Arabidopsis
Regulator
Plant Science
Genes, Plant
ubiquitination
03 medical and health sciences
Gene Expression Regulation, Plant
Plant Cells
flower development
Humans
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
inflorescence
Transcription factor
Post-transcriptional regulation
Leafy
cullin 3
reproductive and urinary physiology
biology
Arabidopsis Proteins
fungi
food and beverages
Meristem
Cullin Proteins
Plants, Genetically Modified
biology.organism_classification
LEAFY
Cell biology
Plant Leaves
Phenotype
030104 developmental biology
Inflorescence
Mutation
BLADE-ON-PETIOLE
Protein Binding
Transcription Factors
post-transcriptional regulation
Subjects
Details
- ISSN :
- 0028646X and 14698137
- Volume :
- 220
- Database :
- OpenAIRE
- Journal :
- New Phytologist
- Accession number :
- edsair.doi.dedup.....ace82d9fb490720551b7ce571ef21454