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HPLC Method and Antioxidant Activity for Bioactive Component Determination of Lycopersicon esculentum Mill. Varieties from a Coastal Area of Southern Spain
- Source :
- Food Analytical Methods. 7:660-668
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- The tomato (Lycopersicon esculentum Mill.) is one of the most widely consumed vegetables and is a component of the so-called “Mediterranean diet.” It represents a significant source of antioxidants in the human diet, with an important biological function (lycopene and β-carotene), as well as other components with antioxidant properties. Antioxidant contents differ according to the variety of tomato, and environmental and agronomic conditions of growth. The aim is to optimize an high-performance liquid chromatography method for the determination of bioactive compounds and to use different antioxidant tests to determine free-radical scavenging activity.The analytical parameters, recovery assays higher than 97 %, satisfactory precision with Relative standard deviation values below 8 %, good linearity (r > 0.999), good sensitivity, and appropriate limits of detection and quantification show that the technique used is satisfactory for measuring these compounds. The tomato samples examined contain highly bioactive components and have high antioxidant capacity with high correlation between phenolic compound contents and antioxidant activity.
- Subjects :
- Antioxidant
medicine.medical_treatment
Relative standard deviation
β-Carotene
Applied Microbiology and Biotechnology
High-performance liquid chromatography
Tomato
Lycopersicon
Analytical Chemistry
chemistry.chemical_compound
Lycopene
medicine
Safety, Risk, Reliability and Quality
Hplc method
Detection limit
Chromatography
biology
Antioxidant capacity
HPLC
food and beverages
biology.organism_classification
chemistry
Safety Research
Food Science
Subjects
Details
- ISSN :
- 1936976X and 19369751
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Food Analytical Methods
- Accession number :
- edsair.doi.dedup.....ad4f2756bfe88b2f6b6c99de1f1293c1