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Effects of cadmium on nuclear protein kinase C
- Source :
- Environmental Health Perspectives
- Publication Year :
- 1994
- Publisher :
- Environmental Health Perspectives, 1994.
-
Abstract
- Cadmium is a carcinogen whose genotoxicity is only weak. Besides its tumor-initiating capacity, cadmium may be tumor-promoting, since it interferes with several steps of cellular signal transduction. We have investigated effects of cadmium(II) on protein kinase C (PKC), which is a key enzyme in the control of cellular growth and differentiation. Tumor-promoting phorbol esters cause an activation and translocation of PKC from the cytosol to the plasma membrane and to the nucleus of mammalian cells. In mouse 3T3/10 T 1/2 fibroblasts, cadmium(II) potentiated the effect of phorbol ester on nuclear binding and activation of PKC. Furthermore, in a reconstituted system consisting of rat liver nuclei and rat brain PKC, cadmium stimulated the binding of the enzyme to a 105-kDa protein. We propose a model in which cadmium(II) substitutes for zinc(II) in the regulatory domain of PKC, thus rendering the putative protein-protein binding site exposed. Further work is required to elucidate the potential role of the nuclear PKC binding protein(s) in the control of cell proliferation.
- Subjects :
- inorganic chemicals
Health, Toxicology and Mutagenesis
chemistry.chemical_element
Plasma protein binding
Biology
medicine.disease_cause
Mice
medicine
Animals
Nuclear protein
Protein Kinase C
Protein kinase C
Carcinogen
Cell Nucleus
Cadmium
Kinase
Public Health, Environmental and Occupational Health
Nuclear Proteins
3T3 Cells
Rats
Zinc
Cell nucleus
medicine.anatomical_structure
Liver
chemistry
Biochemistry
Carcinogens
Genotoxicity
Protein Binding
Research Article
Subjects
Details
- ISSN :
- 15529924 and 00916765
- Volume :
- 102
- Database :
- OpenAIRE
- Journal :
- Environmental Health Perspectives
- Accession number :
- edsair.doi.dedup.....ad67b70aff25faf0cd298a2ca14834cc