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Bread wheat TaSPO11‐1 exhibits evolutionarily conserved function in meiotic recombination across distant plant species
- Source :
- Plant Journal, Plant Journal, Wiley, 2020, 103 (6), pp.2052-2068. ⟨10.1111/tpj.14882⟩, Plant Journal, Wiley, 2020, 103, pp.2052-2068. ⟨10.1111/tpj.14882⟩, Plant Journal, 2020, 103, pp.2052-2068. ⟨10.1111/tpj.14882⟩
- Publication Year :
- 2020
- Publisher :
- HAL CCSD, 2020.
-
Abstract
- International audience; The manipulation of meiotic recombination in crops is essential to develop new plant varieties rapidly, helping to produce more cultivars in a sustainable manner. One option is to control the formation and repair of the meiosis-specific DNA double-strand breaks (DSBs) that initiate recombination between the homologous chromosomes and ultimately lead to crossovers. These DSBs are introduced by the evolutionarily conserved topoisomerase-like protein SPO11 and associated proteins. Here, we characterized the homoeologous copies of the SPO11-1 protein in hexaploid bread wheat (Triticum aestivum). The genome contains three SPO11-1 gene copies that exhibit 93-95% identity at the nucleotide level, and clearly the A and D copies originated from the diploid ancestors Triticum urartu and Aegilops tauschii, respectively. Furthermore, phylogenetic analysis of 105 plant genomes revealed a clear partitioning between monocots and dicots, with the seven main motifs being almost fully conserved, even between clades. The functional similarity of the proteins among monocots was confirmed through complementation analysis of the Oryza sativa (rice) spo11-1 mutant by the wheat TaSPO11-1-5D coding sequence. Also, remarkably, although the wheat and Arabidopsis SPO11-1 proteins share only 55% identity and the partner proteins also differ, the TaSPO11-1-5D cDNA significantly restored the fertility of the Arabidopsis spo11-1 mutant, indicating a robust functional conservation of the SPO11-1 protein activity across distant plants. These successful heterologous complementation assays, using both Arabidopsis and rice hosts, are good surrogates to validate the functionality of candidate genes and cDNA, as well as variant constructs, when the transformation and mutant production in wheat is much longer and more tedious.
- Subjects :
- 0106 biological sciences
0301 basic medicine
[SDV]Life Sciences [q-bio]
Plant Science
[SDV.GEN] Life Sciences [q-bio]/Genetics
01 natural sciences
F30 - Génétique et amélioration des plantes
Arabidopsis
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics
wheat
Conserved Sequence
Triticum
ComputingMilieux_MISCELLANEOUS
Plant Proteins
2. Zero hunger
Genetics
meiotic recombination
biology
phytogénétique
food and beverages
Protéine
Complementation
[SDV] Life Sciences [q-bio]
Meiosis
Ploidy
Méiose
Spo11
Gene Transfer, Horizontal
Aegilops
SPO11-1
Triticum aestivum
[SDV.CAN]Life Sciences [q-bio]/Cancer
Genes, Plant
Evolution, Molecular
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
Aegilops tauschii
protein evolution
Gene
[SDV.GEN]Life Sciences [q-bio]/Genetics
Génome
Arabidopsis Proteins
fungi
Oryza
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology
Cell Biology
biology.organism_classification
[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
030104 developmental biology
Triticum urartu
grasses
biology.protein
Homologous recombination
Sequence Alignment
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 09607412 and 1365313X
- Database :
- OpenAIRE
- Journal :
- Plant Journal, Plant Journal, Wiley, 2020, 103 (6), pp.2052-2068. ⟨10.1111/tpj.14882⟩, Plant Journal, Wiley, 2020, 103, pp.2052-2068. ⟨10.1111/tpj.14882⟩, Plant Journal, 2020, 103, pp.2052-2068. ⟨10.1111/tpj.14882⟩
- Accession number :
- edsair.doi.dedup.....ad6ff56fc8cffae21e9eac3273a9e3bc