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Role of WNT10A-expressing kidney fibroblasts in acute interstitial nephritis
- Source :
- PLoS ONE, Vol 9, Iss 7, p e103240 (2014), PLoS ONE
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- WNT signaling mediates various physiological and pathological processes. We previously showed that WNT10A is a novel angio/stromagenic factor involved in such processes as tumor growth, wound healing and tissue fibrosis. In this study, we investigated the role of WNT10A in promoting the fibrosis that is central to the pathology of acute interstitial nephritis (AIN). We initially asked whether there is an association between kidney function (estimated glomerular filtration rate; eGFR) and WNT10A expression using kidney biopsies from 20 patients with AIN. Interestingly, patients with WNT10A expression had significantly lower eGFR than WNT10A-negative patients. However, changes in kidney function were not related to the level of expression of other WNT family members. Furthermore, there was positive correlation between WNT10A and α-SMA expression. We next investigated the involvement of WNT10A in kidney fibrosis processes using COS1 cells, a kidney fibroblast cell line. WNT10A overexpression increased the level of expression of fibronectin and peroxiredoxin 5. Furthermore, WNT10A overexpression renders cells resistant to apoptosis induced by hydrogen peroxide and high glucose. Collectively, WNT10A may induce kidney fibrosis and associate with kidney dysfunction in AIN.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Clinical Pathology
Renal function
lcsh:Medicine
Apoptosis
Pathology and Laboratory Medicine
Kidney
Diagnostic Medicine
Fibrosis
Molecular Cell Biology
Medicine and Health Sciences
medicine
Humans
lcsh:Science
Aged
Multidisciplinary
biology
business.industry
lcsh:R
Wnt signaling pathway
Biology and Life Sciences
Kidney metabolism
Cell Biology
Fibroblasts
medicine.disease
Actins
Extracellular Matrix
Fibronectins
Wnt Proteins
Fibronectin
Oxidative Stress
medicine.anatomical_structure
Gene Expression Regulation
Nephrology
Acute Disease
biology.protein
Nephritis, Interstitial
lcsh:Q
Cellular Structures and Organelles
business
Wound healing
Nephritis
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....ad9b10c984fc057c221ddcaffe3d5c2c