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Overexpression of CD105 in rat myoblasts: role of CD105 in cell attachment, spreading and survival
- Source :
- Scopus-Elsevier, Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2004
-
Abstract
- 7 p.-5 fig.<br />CD105 is a receptor for transforming growth factor-beta but it is also considered to be involved in cellular functions such as cell adhesion and migration. Using CD105 transfected rat myoblasts, we have investigated the role of CD105 in cell adhesion, spreading, growth and migration. CD105 transfected myoblasts expressed abundant CD105, which was preferentially located within focal adhesion sites. These cells took on a bipolar morphology whereas mock cells remained polygonal or rounded, and when wounded, CD105 expressing cells realigned their long axis prior to migrating and migrated as a cohort of cells. CD105 expression promoted cellular attachment, spreading, survival and growth in serum-free conditions and each of these parameters could be inhibited by a RGD-containing peptide but not a RAD-containing peptide. Mock-transfected cells could not attach, spread or grow under these conditions. Attachment, spreading and growth in CD105 expressing cells could be promoted by the addition of a monoclonal antibody against CD105. Expression of CD105 resulted in the phosphorylation of JNK1 but had no effect on beta1 integrin expression. From this preliminary study, we conclude that in addition to acting as a transforming growth factor-beta receptor, CD105 has an important role in cell adhesion, migration and survival.
- Subjects :
- Cancer Research
Cell signaling
Cell Survival
Cell
Gene Expression
Vascular Cell Adhesion Molecule-1
Receptors, Cell Surface
Biology
Transfection
Cell survival
Focal adhesion
Myoblasts
Antigens, CD
medicine
Cell Adhesion
Animals
Humans
Mitogen-Activated Protein Kinase 8
Phosphorylation
Cell adhesion
Integrin beta1
Endoglin
Adhesion
Cell cycle
Molecular biology
Rats
Up-Regulation
CD105
medicine.anatomical_structure
Oncology
Signal transduction
Cell signalling
Signal Transduction
Subjects
Details
- ISSN :
- 10196439
- Volume :
- 25
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- International journal of oncology
- Accession number :
- edsair.doi.dedup.....adbd3bb7783f0edb68920ff0fb7c1567