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Caveolin- and clathrin-independent entry of BKPyV into primary human proximal tubule epithelial cells
- Source :
- Virology. 492:66-72
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- BK polyomavirus (BKPyV) is a human pathogen that causes polyomavirus-associated nephropathy and hemorrhagic cystitis in transplant patients. Gangliosides and caveolin proteins have previously been reported to be required for BKPyV infection in animal cell models. Recent studies from our lab and others, however, have indicated that the identity of the cells used for infection studies can greatly influence the behavior of the virus. We therefore wished to re-examine BKPyV entry in a physiologically relevant primary cell culture model, human renal proximal tubule epithelial cells. Using siRNA knockdowns, we interfered with expression of UDP-glucose ceramide glucosyltransferase (UGCG), and the endocytic vesicle coat proteins caveolin 1, caveolin 2, and clathrin heavy chain. The results demonstrate that while BKPyV does require gangliosides for efficient infection, it can enter its natural host cells via a caveolin- and clathrin-independent pathway. The results emphasize the importance of studying viruses in a relevant cell culture model.
- Subjects :
- 0301 basic medicine
Monosaccharide Transport Proteins
Caveolin 2
Caveolin 1
Primary Cell Culture
G(M1) Ganglioside
Endocytosis
medicine.disease_cause
Clathrin
Article
Cell Line
Kidney Tubules, Proximal
03 medical and health sciences
Gangliosides
Virology
Caveolin
medicine
Humans
RNA, Small Interfering
biology
Epithelial Cells
Virus Internalization
Cell biology
BK virus
MicroRNAs
030104 developmental biology
Endocytic vesicle
Gene Expression Regulation
Cell culture
BK Virus
Clathrin Heavy Chains
Host-Pathogen Interactions
biology.protein
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 492
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....adcac141f3ca44b77d6a1d1bb4d39fdc