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In vivo real-time assessment of the anastomotic blood supply in colorectal surgery using confocal laser endomicroscopy in an anastomotic model
- Source :
- Surgical Endoscopy. 36:4136-4144
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Anastomotic ischemia can affect healing and eventually lead to anastomotic leakage, and confocal laser endomicroscopy (CLE) can offer detailed observations at the subcellular level. We aimed to evaluate the anastomotic microcirculation in different anastomotic perfusion models using CLE. Anastomotic perfusion models were established using twelve rabbits distributed into two groups: group A (good perfusion, n = 6) and group B (poor perfusion, n = 6). Afterward, intraoperative detection of anastomotic perfusion was carried out using CLE, and quantitative analysis of blood cells was performed. Rabbits that satisfied the criteria underwent a second exploratory operation and specimens were stained by hematoxylin and eosin. Enhanced with fluorescein sodium, capillaries were obviously highlighted in group A, while few capillaries were viewed in group B. Delayed development of fluorescence occurred in group B. The average flow of blood cells was 37.0 ± 5.93 per minute in group A and 6.33 ± 2.16 per minute in group B (p
- Subjects :
- medicine.medical_specialty
Microscopy, Confocal
business.industry
Lasers
Anastomosis, Surgical
fungi
H&E stain
Ischemia
Anastomotic Leak
Anastomosis
medicine.disease
Colorectal surgery
Microcirculation
Animals
Humans
Medicine
Surgery
Rabbits
business
Nuclear medicine
Colorectal Surgery
Perfusion
Digestive System Surgical Procedures
Abdominal surgery
Exploratory surgery
Subjects
Details
- ISSN :
- 14322218 and 09302794
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Surgical Endoscopy
- Accession number :
- edsair.doi.dedup.....adcb921020f6700a7807b04679eb6e92