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Periodontitis induced byPorphyromonas gingivalisdrives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune response
- Source :
- Gut, Gut, BMJ Publishing Group, 2017, 66 (5), pp.872-885. ⟨10.1136/gutjnl-2015-309897⟩, Gut, 2017, 66 (5), pp.872-885. ⟨10.1136/gutjnl-2015-309897⟩
- Publication Year :
- 2016
- Publisher :
- BMJ, 2016.
-
Abstract
- International audience; OBJECTIVE: To identify a causal mechanism responsible for the enhancement of insulin resistance and hyperglycaemia following periodontitis in mice fed a fat-enriched diet. DESIGN: We set-up a unique animal model of periodontitis in C57Bl/6 female mice by infecting the periodontal tissue with specific and alive pathogens like Porphyromonas gingivalis (Pg), Fusobacterium nucleatum and Prevotella intermedia. The mice were then fed with a diabetogenic/non-obesogenic fat-enriched diet for up to 3 months. Alveolar bone loss, periodontal microbiota dysbiosis and features of glucose metabolism were quantified. Eventually, adoptive transfer of cervical (regional) and systemic immune cells was performed to demonstrate the causal role of the cervical immune system. RESULTS: Periodontitis induced a periodontal microbiota dysbiosis without mainly affecting gut microbiota. The disease concomitantly impacted on the regional and systemic immune response impairing glucose metabolism. The transfer of cervical lymph-node cells from infected mice to naive recipients guarded against periodontitis-aggravated metabolic disease. A treatment with inactivated Pg prior to the periodontal infection induced specific antibodies against Pg and protected the mouse from periodontitis-induced dysmetabolism. Finally, a 1-month subcutaneous chronic infusion of low rates of lipopolysaccharides from Pg mimicked the impact of periodontitis on immune and metabolic parameters. CONCLUSIONS: We identified that insulin resistance in the high-fat fed mouse is enhanced by pathogen-induced periodontitis. This is caused by an adaptive immune response specifically directed against pathogens and associated with a periodontal dysbiosis
- Subjects :
- Lipopolysaccharides
0301 basic medicine
Cell Transplantation
[SDV]Life Sciences [q-bio]
Gingiva
Adaptive Immunity
immune response
Mice
Random Allocation
0302 clinical medicine
Bacteroidaceae Infections
Lymphocytes
Gut Microbiota
biology
Microbiota
Vaccination
Gastroenterology
Prevotella intermedia
Acquired immune system
3. Good health
Female
Porphyromonas gingivalis
BACTERIAL PATHOGENESIS
Diet, High-Fat
Microbiology
Interferon-gamma
03 medical and health sciences
Immune system
Insulin resistance
Diabetes Mellitus
medicine
Animals
Periodontitis
Interleukin-6
[SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology
030206 dentistry
biology.organism_classification
medicine.disease
BACTERIAL INFECTION
Diet
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
Hyperglycemia
Immunology
Dysbiosis
Lymph Nodes
Insulin Resistance
Fusobacterium nucleatum
Spleen
Subjects
Details
- ISSN :
- 14683288 and 00175749
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Gut
- Accession number :
- edsair.doi.dedup.....ade10ab19bcbd38aa75d795c2ef7256a